T-NHL TCRγ多重引物基因重排PCR参数优化
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【摘要】目的优化PCR多个参数分析福尔马林固定石蜡包埋标本中T细胞非霍奇金淋巴瘤(T-NHL)的TCRγ多重引物基因重排,以建立PCR扩增中不同变量性能改变的重要意义。方法优化PCR循环参数,采用优化的PCR方法检测53例T-NHL的TCRγ基因重排率。结果53例T-NHL中,采用优化的TCRγ多重引物克隆性TCRγ基因重排检测率为623%。结论实验性的调整PCR参数对FFPE组织的克隆分析,可能会有很大的价值。【关键词】T-NHLTCRγ基因重排PCR
The optimizations of PCR parameter of TCRγ multiple primer gene rearrangement in T-NHLDU Shan-mei,ZHOU Ren,LIU Kui. Shandong Wanjie Medical University,Zibo 255213,China【Abstract】ObjectiveTo optimize multi-parameter of PCR,and analyzed TCRγ gene rearrangement on formalin fixed and paraffin embedded(FFPE)samples in T-NHL,in order to establish the significance of different variables on performance of PCR amplification.MethodsTo change PCR cycle parameters,detecte the rate of the cloning TCRγ gene rearrangement in 53 cases of T-NHL by Using our improved protocol of PCR.ResultsUsing our improved protocol of the TCRγ multi-primers,the detection rate of the cloning TCRγ gene rearrangement in 53 cases of T-NHL was 623% ......
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