慢性乙型病毒性肝炎湿热量化的临床探讨(1)
【摘要】 目的：探讨慢性乙型病毒性肝炎湿热症患者的临床量化规律。方法：选取2012年1月-2013年12月高要市人民医院消化内科住院部西医诊断慢性乙型病毒性肝炎，中医辨证属于湿热证患者101例，将患者分为两组，其中慢性乙型病毒性肝炎湿偏重证者作为A组(50例)，慢性乙型病毒性肝炎热偏重证者作为B组(51例)，同期选取肝功能正常，未合并肝脏疾病和湿热证的健康人群作为正常组(50例)，酶联免疫吸附测定法(ELISA 法)检测尿液水通道蛋白2(AQP-2)、血清热休克蛋白70(HSP70)水平，同时用流式细胞仪检测外周血T淋巴细胞亚群的变化。结果：A、B组AQP-2均明显高于对照组，比较差异均具有统计学意义(P<0.05)，其中A组AQP-2明显高于B组，两组比较差异具有统计学意义(P<0.05)；A、B组HSP70均明显高于对照组，比较差异具有统计学意义(P<0.05)，其中B组HSP70明显高于A组，两组比较差异具有统计学意义(P<0.05)；A、B组CD4+、CD4+/CD8+均明显低于对照组，CD8+均明显高于对照组，比较差异具有统计学意义(P<0.05)，A组和B组CD4+、CD8+、CD4+/CD8+比较差异均无统计学意义(P>0.05)。结论：慢性乙型病毒性肝炎湿热证存在AQP-2、HSP70和CD8+等指标显著增加现象，同时存在CD4+、CD4+/CD8+等指标显著降低现象，其中慢性乙型病毒性肝炎湿偏重证者AQP-2增加程度更明显，热偏重证者HSP70增加程度更明显。
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【关键词】 慢性乙型病毒性肝炎； 湿热证； 量化
Clinical Quantization Study of Chronic Hepatitis B Combined Dampness-heat Syndrome/CHEN Pei-chan，HOU Yan-ping，ZHENG Zhen，et al.//Medical Innovation of China，2015，12(30)：112-115
【Abstract】 Objective：To investigate the clinical quantitative rules of chronic hepatitis B combined dampness-heat syndrome.Method：101 cases of chronic hepatitis B(Western medicine diagnosis) and dampness-heat syndrome inpatients internal medicine Department in People's Hospital of Gaoyao City from January 2012 to December 2013 were divided into two groups，chronic hepatitis B combined tend-to-dampness were selected as A group (50 cases)，chronic hepatitis B combined tend-to- heat were selected as B group (51 cases)， and in the same period，healthy people of normal liver function，unincorporated liver disease and syndrome of dampness heat were selected as normal group (50 cases)， the urine aquaporin 2 (AQP-2) and heat shock protein 70 (heat shock protein 70， HSP70) level were detected by enzyme linked immunosorbent assay (ELISA) method， and peripheral blood T lymphocyte subsets were detected by flow cytometry.Result：AQP-2 of A，B group were significantly higher than that of control group，with significant difference(P<0.05)， AQP-2 of A group was significantly higher than that of B group，with significant differences between the two groups (P<0.05).Plasma HSP 70 of A，B group were significantly higher than that of control group，with significant difference(P<0.05)，plasma HSP70 of B group was significantly higher than that of A group，with significant differences between the two groups (P<0.05).CD4+， CD4+/CD8+ of A，B group were significantly lower than those of control group， CD8+ was significantly higher than that of control group，with significant difference (P<0.05)，there were no significant difference of CD4+，CD8+，CD4+/CD8+ between A group and B group (P>0.05).Conclusion：The quantification indexes(AQP-2，HSP70，CD8+) of chronic hepatitis B with dampness-heat syndrome significantly increase，and quantitative index (CD4+，CD4+/CD8+)decrease，which AQP-2 of tend-to-dampness increase more obviously， HSP70 of tend-to-heat increases more obviously., http://www.100md.com(陈佩婵 侯延平 郑振 刘先秒 谭庆林)
, http://www.100md.com
【关键词】 慢性乙型病毒性肝炎； 湿热证； 量化
Clinical Quantization Study of Chronic Hepatitis B Combined Dampness-heat Syndrome/CHEN Pei-chan，HOU Yan-ping，ZHENG Zhen，et al.//Medical Innovation of China，2015，12(30)：112-115
【Abstract】 Objective：To investigate the clinical quantitative rules of chronic hepatitis B combined dampness-heat syndrome.Method：101 cases of chronic hepatitis B(Western medicine diagnosis) and dampness-heat syndrome inpatients internal medicine Department in People's Hospital of Gaoyao City from January 2012 to December 2013 were divided into two groups，chronic hepatitis B combined tend-to-dampness were selected as A group (50 cases)，chronic hepatitis B combined tend-to- heat were selected as B group (51 cases)， and in the same period，healthy people of normal liver function，unincorporated liver disease and syndrome of dampness heat were selected as normal group (50 cases)， the urine aquaporin 2 (AQP-2) and heat shock protein 70 (heat shock protein 70， HSP70) level were detected by enzyme linked immunosorbent assay (ELISA) method， and peripheral blood T lymphocyte subsets were detected by flow cytometry.Result：AQP-2 of A，B group were significantly higher than that of control group，with significant difference(P<0.05)， AQP-2 of A group was significantly higher than that of B group，with significant differences between the two groups (P<0.05).Plasma HSP 70 of A，B group were significantly higher than that of control group，with significant difference(P<0.05)，plasma HSP70 of B group was significantly higher than that of A group，with significant differences between the two groups (P<0.05).CD4+， CD4+/CD8+ of A，B group were significantly lower than those of control group， CD8+ was significantly higher than that of control group，with significant difference (P<0.05)，there were no significant difference of CD4+，CD8+，CD4+/CD8+ between A group and B group (P>0.05).Conclusion：The quantification indexes(AQP-2，HSP70，CD8+) of chronic hepatitis B with dampness-heat syndrome significantly increase，and quantitative index (CD4+，CD4+/CD8+)decrease，which AQP-2 of tend-to-dampness increase more obviously， HSP70 of tend-to-heat increases more obviously., http://www.100md.com(陈佩婵 侯延平 郑振 刘先秒 谭庆林)