FK1706促进外周神经旁路移植术后大鼠神经再生的研究(1)
【摘要】 目的：探究在大鼠神经旁路移植术后应用FK1706对促进神经再生的作用。方法：SD雄性成年大鼠20只，随机分成两组，每组10只，行左坐骨神经开窗离断-自体桡神经旁路移植术。实验组(FK1706组)术后即日开始连续8周左下肢局部肌注FK1706(0.32 mg/kg)，对照组做空白对照。8周后行左侧坐骨神经电生理监测、左腓肠肌肌肉湿重及肌纤维横截面积测定。结果：8周后，实验组的CAMP波幅高于对照组，MNCV快于对照组，差异均有统计学意义(P<0.01)。实验组的腓肠肌湿重及肌纤维面积均高于对照组，差异均有统计学意义(P<0.05)。两组的近端有髓神经纤维数比较，差异无统计学意义(P>0.05)，实验组远端有髓神经纤维数、纤维横截面积均明显优于对照组，差异均有统计学意义(P<0.01)。结论：大鼠神经旁路移植术后应用FK1706能够移植神经再生。
【关键词】 FK1706； 自体神经移植； 神经再生
Study on Effect of FK1706 to Accelerate Neuroregeneration after Nerve Bypass Grafting Surgery/XIAO Yuhua，XU Jie，LI Jun.//Medical Innovation of China，2018，15(13)：023-026
, 百拇医药
【Abstract】 Objective：To investigate the effects of FK1706 to neuroregeneration after nerve bypass grafting.Method：20 adult male SD rats were randomly divided into two groups，10 cases in each group，the left sciatic nerve fenestration and autologous radial nerve bypass grafting was performed.In the experimental group，FK1706(0.32 mg/kg) was injected into the left lower extremities for 8 weeks immediately after the operation，the control group was made blank control.After 8 weeks，the electrophysiological monitoring of the left sciatic nerve，the wet weight of the left gastrocnemius muscle and the cross section of the muscle fiber were measured.Result：After 8 weeks，the amplitude of CAMP in the experimental group was higher than that in the control group，MNCV was faster than that of control group，the differences were statistically significant(P<0.01).The wet weight and fiber area of gastrocnemius in the experimental group were higher than those in the control group(P<0.05).There was no significant difference in the number of myelinated nerve fibers between the two groups(P>0.05)，the number and cross-sectional area of distal myelinated nerve fibers in the experimental group were significantly better than those in the control group，the differences were statistically significant(P<0.01).Conclusion：The effects of FK1706 to accelerate neuroregeneration after nerve bypass grafting surgery is siginicant.
, 百拇医药
【Key words】 FK1706； Autogenous nerve transplantation； Neuroregeneration
First-author’s address：Fujian Provincial Hospital，Fuzhou 350001，China
doi：10.3969/j.issn.1674-4985.2018.13.006
神经旁路移植术在临床上可用于治疗有神经瘤生成的周围神经损伤，该手术方式既保留了神经原有的功能，还增加了动力神经[1]。实验报道肯定了神经旁路移植术后移植神经再生的现象。临床上周围神经再生速度1～2 mm/d，修复效果欠佳，因此，希望找到一种促进神经再生且副作用小的药物。
已被FDA认证的FK506是一种免疫抑制性药物[2]。FK506在广泛应用于器官移植后免疫抑制的领域中发现其具有促进神经生长的作用[3]，但FK506本身对人体组织器官具有强效免疫抑制作用，这大大局限了FK506在神经损伤后治疗作用。FK1706作为FK506的衍生物在2005年首先被报道[4]，其保留了促神经生长的作用，很大程度上弱化了FK506的免疫抑制作用。有文献报道，FK1706能够促进轴突再生的基因转录和表达[5]。本研究建立大鼠坐骨神经损伤模型，肌注FK1706药物进行干預，以评价其促进神经再生的作用。, 百拇医药(肖毓华 徐杰 李鋆)
【关键词】 FK1706； 自体神经移植； 神经再生
Study on Effect of FK1706 to Accelerate Neuroregeneration after Nerve Bypass Grafting Surgery/XIAO Yuhua，XU Jie，LI Jun.//Medical Innovation of China，2018，15(13)：023-026
, 百拇医药
【Abstract】 Objective：To investigate the effects of FK1706 to neuroregeneration after nerve bypass grafting.Method：20 adult male SD rats were randomly divided into two groups，10 cases in each group，the left sciatic nerve fenestration and autologous radial nerve bypass grafting was performed.In the experimental group，FK1706(0.32 mg/kg) was injected into the left lower extremities for 8 weeks immediately after the operation，the control group was made blank control.After 8 weeks，the electrophysiological monitoring of the left sciatic nerve，the wet weight of the left gastrocnemius muscle and the cross section of the muscle fiber were measured.Result：After 8 weeks，the amplitude of CAMP in the experimental group was higher than that in the control group，MNCV was faster than that of control group，the differences were statistically significant(P<0.01).The wet weight and fiber area of gastrocnemius in the experimental group were higher than those in the control group(P<0.05).There was no significant difference in the number of myelinated nerve fibers between the two groups(P>0.05)，the number and cross-sectional area of distal myelinated nerve fibers in the experimental group were significantly better than those in the control group，the differences were statistically significant(P<0.01).Conclusion：The effects of FK1706 to accelerate neuroregeneration after nerve bypass grafting surgery is siginicant.
, 百拇医药
【Key words】 FK1706； Autogenous nerve transplantation； Neuroregeneration
First-author’s address：Fujian Provincial Hospital，Fuzhou 350001，China
doi：10.3969/j.issn.1674-4985.2018.13.006
神经旁路移植术在临床上可用于治疗有神经瘤生成的周围神经损伤，该手术方式既保留了神经原有的功能，还增加了动力神经[1]。实验报道肯定了神经旁路移植术后移植神经再生的现象。临床上周围神经再生速度1～2 mm/d，修复效果欠佳，因此，希望找到一种促进神经再生且副作用小的药物。
已被FDA认证的FK506是一种免疫抑制性药物[2]。FK506在广泛应用于器官移植后免疫抑制的领域中发现其具有促进神经生长的作用[3]，但FK506本身对人体组织器官具有强效免疫抑制作用，这大大局限了FK506在神经损伤后治疗作用。FK1706作为FK506的衍生物在2005年首先被报道[4]，其保留了促神经生长的作用，很大程度上弱化了FK506的免疫抑制作用。有文献报道，FK1706能够促进轴突再生的基因转录和表达[5]。本研究建立大鼠坐骨神经损伤模型，肌注FK1706药物进行干預，以评价其促进神经再生的作用。, 百拇医药(肖毓华 徐杰 李鋆)