苦参素对肝癌细胞HepG2增殖及能量代谢的影响(1)
【摘要】 目的：观察苦参素(OM)对人肝癌细胞株HepG2细胞增殖、细胞内ATP含量、上清液乳酸生成速率的影响，探讨其可能的机制。方法：体外培养人肝癌细胞株HepG2，CCK-8法观察不同浓度、不同作用时间的OM对HepG2细胞增殖的影响；高效液相色谱技术、乳酸试剂盒分别检测苦参素作用48 h后细胞内ATP、上清液乳酸乳酸生成速率影响。结果：OM可以抑制人肝癌细胞株HepG2细胞增殖，其作用效果随药物浓度、作用时间增加而增强(P<0.05)；不同浓度苦参素作用48 h后细胞内ATP含量、上清液乳酸生成速率显著下降(P<0.05)。结论：OM可以抑制人肝癌细胞株HepG2细胞增殖，呈现时间-剂量依赖性，作用机制可能是抑制细胞有氧糖酵解使细胞内ATP含量下降有关。
【关键词】 肝癌细胞株HepG2； 苦参素； 三磷酸腺苷； 糖酵解
Influence of Oxymatrine on Proliferation and Energy Metabolise of HepG2 Hepatoma Cell/JIANG Qi，HUANG Zansong，SU Jianwei，et al.//Medical Innovation of China，2018，15(13)：027-030
, http://www.100md.com
【Abstract】 Objective：To observe the effects of oxymatrine(OM) on the proliferation、The level of intracellular ATP and Lactic acid concentration in the culture supernatant of HepG2 hepatoma cell，and to disscuss potential mechanism.Method：HepG2 hepatoma cell was cultured in vitro，CCK-8 method was used to detect the proliferation effect at the different concentrations and different times；a high performance liquid chromatography and enzymatic colorimetric method were used to measure the levels of intracellular ATP and the rate of lactic acid concentration growth in the culture supernatant after treating with 48 h oxymatrine respectively.Result：There was significant difference in inhibited effect of OM on the proliferation of HepG2 hepatoma cell，and with the increase of OM concentration and prolonging the time of action， the inhibition effect of OM on the human hepatoma cell line HepG2 was more obvious(P<0.05).The level of intracellular ATP lower and the rate of Lactic acid concentration growth in the culture supernatant After treating with 48 h OM by the different concentrations(P<0.05).Conclusion：OM can inhibit the proliferation of HepG2 hepatoma cell，and there is time and dose depended.The mechanism of OM anti-hepatoma may be that through targeting of the glycolytic pathway and results in decreased ATP.
, 百拇医药
【Key words】 HepG2 hepatoma cell； Oxymatrine； Adenosine triphosphate； Glycolysis
First-author’s address：Affiliated Hospital of Youjiang Medical University for Nationalities，Baise 533000，China
doi：10.3969/j.issn.1674-4985.2018.13.007
有氧糖酵解即“Warburg effect”參与肝癌的发生、发展及转移成为研究的热点，基于肿瘤细胞能量代谢为靶点的药物，有望成为恶性肿瘤临床治疗的新的突破口[1-3]。前期实验研究表明苦参素可能通过上调MicoRNA122[4]，从而抑制肝癌HepG2细胞增殖。近来研究发现MircoRNA是关键的代谢调节剂，直接或间接参与癌症的代谢。因此研究苦参素对HepG2细胞能量代谢影响，进一步研究其抗肿瘤作用。目前研究苦参素影响肿瘤细胞能量较少。本实验研究苦参素(Oxymatrine或OM)对HepG2细胞增殖及细胞内ATP含量影响，现报道如下。
1 材料与方法
1.1 材料 HepG2人肝癌细胞株：购于中科院上海细胞库；DMEM无糖培养基(GFM)、DMEM低糖培养基(GM)：购于美国GIBICO；胰酶-EDTA混合液、RPMI-1640培养液：购于北京索莱宝科技有限公司；CCK-8试剂盒：购于碧云天生物科技；苦参素注射液：购于正大天晴药业股份有限公司；三磷酸腺苷(ATP)购于美国Sigma公司，HPLC级；抗霉素-A(AntA)美国Sigma公司。仪器：CO2恒温培养箱MCO-18AIC、戴安U3000型液相色谱仪、雷磁PHS-3C PH计、乳酸测定试剂盒, 百拇医药(蒋旗 黄赞松 苏建伟 曹聪)
【关键词】 肝癌细胞株HepG2； 苦参素； 三磷酸腺苷； 糖酵解
Influence of Oxymatrine on Proliferation and Energy Metabolise of HepG2 Hepatoma Cell/JIANG Qi，HUANG Zansong，SU Jianwei，et al.//Medical Innovation of China，2018，15(13)：027-030
, http://www.100md.com
【Abstract】 Objective：To observe the effects of oxymatrine(OM) on the proliferation、The level of intracellular ATP and Lactic acid concentration in the culture supernatant of HepG2 hepatoma cell，and to disscuss potential mechanism.Method：HepG2 hepatoma cell was cultured in vitro，CCK-8 method was used to detect the proliferation effect at the different concentrations and different times；a high performance liquid chromatography and enzymatic colorimetric method were used to measure the levels of intracellular ATP and the rate of lactic acid concentration growth in the culture supernatant after treating with 48 h oxymatrine respectively.Result：There was significant difference in inhibited effect of OM on the proliferation of HepG2 hepatoma cell，and with the increase of OM concentration and prolonging the time of action， the inhibition effect of OM on the human hepatoma cell line HepG2 was more obvious(P<0.05).The level of intracellular ATP lower and the rate of Lactic acid concentration growth in the culture supernatant After treating with 48 h OM by the different concentrations(P<0.05).Conclusion：OM can inhibit the proliferation of HepG2 hepatoma cell，and there is time and dose depended.The mechanism of OM anti-hepatoma may be that through targeting of the glycolytic pathway and results in decreased ATP.
, 百拇医药
【Key words】 HepG2 hepatoma cell； Oxymatrine； Adenosine triphosphate； Glycolysis
First-author’s address：Affiliated Hospital of Youjiang Medical University for Nationalities，Baise 533000，China
doi：10.3969/j.issn.1674-4985.2018.13.007
有氧糖酵解即“Warburg effect”參与肝癌的发生、发展及转移成为研究的热点，基于肿瘤细胞能量代谢为靶点的药物，有望成为恶性肿瘤临床治疗的新的突破口[1-3]。前期实验研究表明苦参素可能通过上调MicoRNA122[4]，从而抑制肝癌HepG2细胞增殖。近来研究发现MircoRNA是关键的代谢调节剂，直接或间接参与癌症的代谢。因此研究苦参素对HepG2细胞能量代谢影响，进一步研究其抗肿瘤作用。目前研究苦参素影响肿瘤细胞能量较少。本实验研究苦参素(Oxymatrine或OM)对HepG2细胞增殖及细胞内ATP含量影响，现报道如下。
1 材料与方法
1.1 材料 HepG2人肝癌细胞株：购于中科院上海细胞库；DMEM无糖培养基(GFM)、DMEM低糖培养基(GM)：购于美国GIBICO；胰酶-EDTA混合液、RPMI-1640培养液：购于北京索莱宝科技有限公司；CCK-8试剂盒：购于碧云天生物科技；苦参素注射液：购于正大天晴药业股份有限公司；三磷酸腺苷(ATP)购于美国Sigma公司，HPLC级；抗霉素-A(AntA)美国Sigma公司。仪器：CO2恒温培养箱MCO-18AIC、戴安U3000型液相色谱仪、雷磁PHS-3C PH计、乳酸测定试剂盒, 百拇医药(蒋旗 黄赞松 苏建伟 曹聪)