【摘要】 目的：比較实时荧光PCR探针法和DNA测序法检测细胞色素氧化酶P450(CYP2C9)和维生素K环氧化物还原酶复合体1(VKORC1)基因多态性的一致性，建立适用于临床推广的华法林基因多态性检测的方法。方法：本研究随机收集清远市人民医院20例就诊患者的全血标本，应用实时荧光PCR探针法和DNA测序法检测其CYP2C9和VKORC1基因的多态性，结果运用Excel软件统计并进行比较分析。结果：两种方法对CYP2C9和VKORC1基因多态性的检测结果一致，且实时荧光PCR探针法比DNA测序法更简易、经济、快捷。结论：与DNA测序法相比，应用实时荧光PCR探针法检测CYP2C9和VKORC1基因的突变具有较高的临床应用价值，比较适用于临床检测。

    【关键词】 实时荧光PCR探针法； DNA测序法； CYP2C9； VKORC1

    【Abstract】 Objective：To compare the polymorphism of cytochrome oxidase P450(CYP2C9) and vitamin K epoxide reductase complex 1(VKORC1) gene by real-time fluorescent PCR and DNA sequencing.Methods of clinical promotion of warfarin gene polymorphism.Method：The whole blood samples of 20 patients in Qingyuan People’s Hospital were randomly collected.The polymorphisms of CYP2C9 and VKORC1 genes were detected by real-time fluorescent PCR and DNA sequencing.The results were analyzed by Excel software and compared.

    Result：The results of two methods were consistent with the detection of CYP2C9 and VKORC1 gene polymorphism ......