石菖蒲有效成分对APP/PS1双转基因小鼠GAP-43表达的影响(1)
【摘要】 目的：觀察石菖蒲有效成分β-细辛醚对APP/PS1双转基因小鼠生长相关蛋白43(growth-associated protein 43，GAP-43)表达的影响，探讨β-细辛醚对APP/PS1双转基因小鼠神经突触可塑性的保护作用及对阿尔茨海默病(AD)的治疗作用机制。方法：选取2019年3-4月40只2月龄APP/PS1双转基因小鼠随机分为模型组，盐酸多奈哌齐组，低剂量组及高剂量组，每组10只，选用10只相同月龄C57BL/6小鼠为空白对照组。空白对照组与模型组分别给予0.1 mL/10 g 0.9%氯化钠溶液，盐酸多奈哌齐组给予0.33 mg/(kg·d)盐酸多奈哌齐，低剂量组给予β-细辛醚25 mg/(kg·d)，高剂量组给予50 mg/(kg·d)β-细辛醚，五组均连续灌胃4周。采用Morris水迷宫法检测并比较各组学习记忆能力，采用免疫荧光法检测并比较各组海马区GAP-43蛋白的表达变化，采用RT-PCR技术检测并比较各组海马区GAP-43mRNA表达水平。结果：模型组、盐酸多奈哌齐组、低剂量组与高剂量组逃避潜伏期均高于空白对照组，且跨越平台次数均低于空白对照组(P<0.05)。盐酸多奈哌齐组、低剂量组与高剂量组逃避潜伏期均低于模型组，且跨越平台次数高于模型组(P<0.05)。盐酸多奈哌齐组与高剂量组逃避潜伏期均低于低剂量组(P<0.05)。盐酸多奈哌齐组与高剂量组跨越平台次数均高于低剂量组，且高剂量组高于盐酸多奈哌齐组(P<0.05)。模型组、盐酸多吗哌齐组、低剂量组与高剂量组GAP-43蛋白与GAP-43mRNA表达量均高于空白对照组(P<0.05)。盐酸多奈哌齐组、低剂量组与高剂量组GAP-43蛋白与GAP-43mRNA表达量均低于模型组(P<0.05)。结论：β-细辛醚具有改善AD的作用，并且作用机制与调控小鼠海马神经突触可塑性相关因子GAP-43的表达相关。
【关键词】 阿尔茨海默病 β-细辛醚 APP/PS1双转基因小鼠 神经生长相关蛋白43
Effects of Active Ingredients of Acorus Tatarindii on GAP-43 Expression in APP/PS1 Double Transgenic Mice/SUN Xiaoxue， WANG Ningning， LI Lin， RONG Hua. //Medical Innovation of China， 2020， 17(25)： 0-025
[Abstract] Objective： To observe the effect of β-asarone on the expression of growth-associated protein 43 (GAP-43) in APP / PS1 double transgenic mice， and to explore the protective effect of β-asarone on the synaptic plasticity of APP / PS1 double transgenic mice and the therapeutic mechanism of β-asarone on Alzheimer’s disease (AD). Method： From March to April 2019， a total of 40 APP/PS1 double transgenic mice with 2 month old were randomly divided into model group， Donepezil Hydrochloride group， low-dose group and high-dose group， with 10 mice in each group. 10 mice with C57BL/6 in the same month age were selected as blank control group. The blank control group and model group were given 0.1 mL/10 g 0.9% sodium chloride solution. The Donepezil Hydrochloride group was given 0.33 mg/(kg·d) Donepezil Hydrochloride. The Low-dose group was given 25 mg/(kg·d) β-asarone. The high-dose group was given 50 mg/(kg·d) β-asarone. All the five groups were given gavage for 4 weeks. The learning and memory ability of each group were detected and compared by Morris water maze method. The expression of GAP-43 protein of each group were detected and compared by immunofluorescence method. The expression of GAP-43 mRNA in hippocampus of each group were detected and compared by RT-PCR. Result： The escape latency of model group， the Donepezil Hydrochloride group， low-dose group and high-dose group were higher than that of blank control group， and the number of crossing the platform were lower than that of the blank control group (P<0.05). The escape latency of the Donepezil Hydrochloride group， low-dose group and high-dose group were lower than that of model group， and the number of crossing the platform were higher than that of model group (P<0.05). The escape latency of the Donepezil Hydrochloride group and the high-dose group were lower than that of low-dose group (P<0.05). The number of crossing the platform in the Donepezil Hydrochloride group and the high dose group were higher than those in the low-dose group， and the high dose group was higher than the Donepezil Hydrochloride group (P<0.05). The expression levels of GAP-43 protein and GAP-43 mRNA in the model group， Domperidone Hydrochloride group， low-dose group and high-dose group were higher than those in the blank control group (P<0.05). The expressions of GAP-43 protein and GAP-43 mRNA in the Donepezil Hydrochloride group， low-dose group and high-dose group were lower than those in the model group (P<0.05). Conclusion： β-asarone has the effect of improving AD， and the mechanism of action is related to the regulation of the expression of GAP-43， a synaptic plasticity-related factor in hippocampus of mice., http://www.100md.com(孙晓雪 王宁宁 李琳 荣华)
【关键词】 阿尔茨海默病 β-细辛醚 APP/PS1双转基因小鼠 神经生长相关蛋白43
Effects of Active Ingredients of Acorus Tatarindii on GAP-43 Expression in APP/PS1 Double Transgenic Mice/SUN Xiaoxue， WANG Ningning， LI Lin， RONG Hua. //Medical Innovation of China， 2020， 17(25)： 0-025
[Abstract] Objective： To observe the effect of β-asarone on the expression of growth-associated protein 43 (GAP-43) in APP / PS1 double transgenic mice， and to explore the protective effect of β-asarone on the synaptic plasticity of APP / PS1 double transgenic mice and the therapeutic mechanism of β-asarone on Alzheimer’s disease (AD). Method： From March to April 2019， a total of 40 APP/PS1 double transgenic mice with 2 month old were randomly divided into model group， Donepezil Hydrochloride group， low-dose group and high-dose group， with 10 mice in each group. 10 mice with C57BL/6 in the same month age were selected as blank control group. The blank control group and model group were given 0.1 mL/10 g 0.9% sodium chloride solution. The Donepezil Hydrochloride group was given 0.33 mg/(kg·d) Donepezil Hydrochloride. The Low-dose group was given 25 mg/(kg·d) β-asarone. The high-dose group was given 50 mg/(kg·d) β-asarone. All the five groups were given gavage for 4 weeks. The learning and memory ability of each group were detected and compared by Morris water maze method. The expression of GAP-43 protein of each group were detected and compared by immunofluorescence method. The expression of GAP-43 mRNA in hippocampus of each group were detected and compared by RT-PCR. Result： The escape latency of model group， the Donepezil Hydrochloride group， low-dose group and high-dose group were higher than that of blank control group， and the number of crossing the platform were lower than that of the blank control group (P<0.05). The escape latency of the Donepezil Hydrochloride group， low-dose group and high-dose group were lower than that of model group， and the number of crossing the platform were higher than that of model group (P<0.05). The escape latency of the Donepezil Hydrochloride group and the high-dose group were lower than that of low-dose group (P<0.05). The number of crossing the platform in the Donepezil Hydrochloride group and the high dose group were higher than those in the low-dose group， and the high dose group was higher than the Donepezil Hydrochloride group (P<0.05). The expression levels of GAP-43 protein and GAP-43 mRNA in the model group， Domperidone Hydrochloride group， low-dose group and high-dose group were higher than those in the blank control group (P<0.05). The expressions of GAP-43 protein and GAP-43 mRNA in the Donepezil Hydrochloride group， low-dose group and high-dose group were lower than those in the model group (P<0.05). Conclusion： β-asarone has the effect of improving AD， and the mechanism of action is related to the regulation of the expression of GAP-43， a synaptic plasticity-related factor in hippocampus of mice., http://www.100md.com(孙晓雪 王宁宁 李琳 荣华)