吴成成 罗文兵 肖慧荣 谢昌营 胡丽霞 崔曼曼 张磊昌

    【摘要】 目的：基于TLR4/NF-κB探讨“金汁”粪菌移植(FMT)干预溃疡性结肠炎(UC)小鼠的机制。方法：选取29只野生型雄性清洁级C57BL/10小鼠为研究对象。随机选取7只作为对照组，其余22只大鼠采用葡聚糖酸钠诱导法建立野生型小鼠UC模型。将这22只大鼠分为造模对照组(n=12)和FMT干预组(n=10)，分别给予PBS液、粪菌液灌肠干预处理。另选取5只野生型雄性清洁级C57BL/10小鼠作为粪菌移植供体来源。观察三组小鼠结肠组织TLR4、NF-κB(p65)表达水平，观察三组小鼠结肠组织中下游细胞因子表达水平，分析TLR4、NF-κB(p65)及下游各细胞因子水平与疾病活动指数(DAI)和组织病理评分(HPS)的关系。结果：干预前，对照组DAI低于造模对照组和FMT干预组(P0.05)。干预后，造模对照组DAI高于对照组和FMT干预组，FMT干预组高于对照组(P0.05). After intervention， DAI in modeling control group was higher than those of the control group and FMT intervention group， while FMT intervention group was higher than that of the control group (P<0.05). DAI in FMT intervention group after intervention was lower than that before intervention (P<0.05). The HPS of the modeling control group was higher than those of the control group and the FMT intervention group ......