蛋白片段互补方法及其在药物筛选中的应用(1)
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【摘要】本文简述了蛋白片段互补及功能互补(protein fragment complementation assay：PCA)。将报告蛋白分割成两个没有功能的片段，分别与两个待检测的蛋白质融合，如果待检测的两个蛋白质能够发生相互作用，就可以使报告蛋白的两个片段发生互补，从而使其功能得以重建。该技术对药物发现及筛选起了巨大的作用，尤其适用于高通量药物筛选。
【关键词】片段功能互补；蛋白质相互作用；药物筛选
Protein fragment complementation method and its application in drug screening ZHOU Qi.Yaan Vocational College,Yaan 625000,China
【Abstract】We described a experimental strategies-protein fragment complementation assays(PCAs) which can be used to detect protein-protein interactions.PCA-based strategies can be used with or instead of traditional target-based drug discovery strategies to identify novel pathway-component proteins of therapeutic interest,it can increase the quantity and quality of information about the actions of potential drugs and to gain insight into the Intricate networks that make up the molecular machinery of living cells.
【Key words】Fragment complementation;Protein-protein interaction;Drug screening
蛋白质控制和调节细胞分裂增殖以及癌性转化等诸多的生命活动 ......
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