OviductusRanaemethodofextractionandisolationofactiveingredien(2)
Wang Li li[10] obtained cholest-5-en-3β,7β-diol and 3β-hydroxy-cholest-5- en-7-one from Oviductus Ranae for the first time. The process was that Oviductus Ranae were pre-extracted with supercritical carbon dioxide (for 4.5h, at 45℃, under 35MPa, 55mL/100g ) to remove the lipin components, then the degreasing Oviductus Ranae were extracted with 85% ethanol. Subsequently, the dried ethanol extracted residue was dissolved in deionised water and extractedwith ethyl acetate, and then the ethyl acetate extraction was concentrated and applied onto columns of sephadex LH-20(mobile phase:chloroform- methano 1:1) and ODS(mobile phase: 90% (v/v) methano) repeately to gave crude products, which were purified by preparative HPLC.
, http://www.100md.com
Column chromatographies of gel and ODS have been widely used in studies on the chemical constituents of traditional chinese medicine.Gel column chromatography rests on varying molecule and fits to the chemical components isolation of vare medicines because of easy operation, fine separation, low adsorption rate to sample. The basis of separating and purifying chemical components of ODS column chromatography is varing molecular polarity. The main features of this method are easy operation and low adsorption rate to sample, so this method is also applied to research of chemical ingredients of vare medicines.
, 百拇医药
3Amides
Wang Yong sheng[5] got hexadecanamide from petroleum ether extraction of Oviductus Ranae by silica column chromatography(mobile phase: petroleum ether-ethyl acetate), recrystallization(petroleum ether-ethanol and acetone ). Lu Li ming[3] also obtained hexadecanamide with supercritical carbon dioxide combining column chromatographies of silica gel(mobile phase: petroleum ether-chloroform and petroleum ether- ethyl acetate ), gel, AgNO3 gel(mobile phase: chloroform- acetone).
, 百拇医药
4Polysaccharide
Polysaccharide can strengthen the immunity, it can not only enhance meshy endodermis system to gobble up a function, but slao increase the amount of immuglobulin. Polysaccharide is effective on tumor and cardiovascular diseases[11].
Polysaccharide were separated form Oviductus Ranae showing positive reactions to Fehling and Molish. The method was thatOviductus Ranae was extracted with water decocting and separated with ion-exchange process. The result of quantitative determination was that the content of reducing suger and polysaccharide were 6.8% and 6.18% in Oviductus Ranae.
, 百拇医药
5Hydantions
1-methylhydantion had the extremelyeffect ofrelieving cough[13].
Wang Yong sheng[13] got 1-methylhydantion from Oviductus Ranae for the first time. The method was that Oviductus Ranae was successively extracted by petrol ether, ethyl acetate and ethanol, subsequently, the ethanol extraction was separated with silica column chromatography(mobile phase: petrol ether- ethyl acetate) and purified with recrystallization(petroleum ether- chloroform).
, http://www.100md.com
6Conclusion
At present, the stuty on Oviductus Ranae has many difficulties, such as the complex chemical ingredients, the low content of active components and the rarely reports about effective ingredients in Oviductus Ranae. So the basic research of Oviductus Ranae is more weaker than that of other valuable chinese medicines. But the wide application of gel column chromatography, ODS column chromatography, microwave extraction, semi-bionic extraction (SBE), CO2-SFE, ultrafiltration and high-speed countercurrent chromatography provides advantage for obtaining high purity active ingredients form Oviductus Ranae, so the extraction and isolation of the active ingredients in Oviductus Ranae is yet to be further studied., http://www.100md.com(WANG Li-li ZHANG Lan-tong WANG Yong-shen)
, http://www.100md.com
Column chromatographies of gel and ODS have been widely used in studies on the chemical constituents of traditional chinese medicine.Gel column chromatography rests on varying molecule and fits to the chemical components isolation of vare medicines because of easy operation, fine separation, low adsorption rate to sample. The basis of separating and purifying chemical components of ODS column chromatography is varing molecular polarity. The main features of this method are easy operation and low adsorption rate to sample, so this method is also applied to research of chemical ingredients of vare medicines.
, 百拇医药
3Amides
Wang Yong sheng[5] got hexadecanamide from petroleum ether extraction of Oviductus Ranae by silica column chromatography(mobile phase: petroleum ether-ethyl acetate), recrystallization(petroleum ether-ethanol and acetone ). Lu Li ming[3] also obtained hexadecanamide with supercritical carbon dioxide combining column chromatographies of silica gel(mobile phase: petroleum ether-chloroform and petroleum ether- ethyl acetate ), gel, AgNO3 gel(mobile phase: chloroform- acetone).
, 百拇医药
4Polysaccharide
Polysaccharide can strengthen the immunity, it can not only enhance meshy endodermis system to gobble up a function, but slao increase the amount of immuglobulin. Polysaccharide is effective on tumor and cardiovascular diseases[11].
Polysaccharide were separated form Oviductus Ranae showing positive reactions to Fehling and Molish. The method was thatOviductus Ranae was extracted with water decocting and separated with ion-exchange process. The result of quantitative determination was that the content of reducing suger and polysaccharide were 6.8% and 6.18% in Oviductus Ranae.
, 百拇医药
5Hydantions
1-methylhydantion had the extremelyeffect ofrelieving cough[13].
Wang Yong sheng[13] got 1-methylhydantion from Oviductus Ranae for the first time. The method was that Oviductus Ranae was successively extracted by petrol ether, ethyl acetate and ethanol, subsequently, the ethanol extraction was separated with silica column chromatography(mobile phase: petrol ether- ethyl acetate) and purified with recrystallization(petroleum ether- chloroform).
, http://www.100md.com
6Conclusion
At present, the stuty on Oviductus Ranae has many difficulties, such as the complex chemical ingredients, the low content of active components and the rarely reports about effective ingredients in Oviductus Ranae. So the basic research of Oviductus Ranae is more weaker than that of other valuable chinese medicines. But the wide application of gel column chromatography, ODS column chromatography, microwave extraction, semi-bionic extraction (SBE), CO2-SFE, ultrafiltration and high-speed countercurrent chromatography provides advantage for obtaining high purity active ingredients form Oviductus Ranae, so the extraction and isolation of the active ingredients in Oviductus Ranae is yet to be further studied., http://www.100md.com(WANG Li-li ZHANG Lan-tong WANG Yong-shen)