HPLC法测定盐酸二甲双胍片的含量
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[摘要] 目的 建立测定盐酸二甲双胍片含量的方法。 方法 采用HPLC法，C18柱(250 mm×6.0 mm，5μm)；流动相为0.01 mol/L磷酸二氢钾缓冲液(内含0.015 mol/L高氯酸，pH为3.0)；检测波长为218 nm。 结果 盐酸二甲双胍在8.07 ~80.74μg/mL浓度范围内线性关系良好(r=0.999 1)，平均回收率为98.02%，RSD=0.76%。 结论 本方法简便、快速、准确，专属性强，可作为盐酸二甲双胍片的含量测定方法。
[关键词] 高效液相色谱法；盐酸二甲双胍片；含量
[中图分类号] R927.2 [文献标识码] B [文章编号] 2095-0616(2011)23-113-02
Content determination of metformin hydrochloride tablets by HPLC
CHEN Yupu
Institute for Food and Drug Control of Jiaozuo City in Henan Province,Jiaozuo 454000,China
[Abstract] Objective To establish a method to determine the content of metforminhy drochloride tablets. Methods HPLC instrument with C18 chromatographic column(250 mm×6.0 mm，5μm)was used .The mobile phase consisted of 0.01 mol/L postassium dihydrogen phosphate and 0.015 mol/L perchloric acid;The wavelength for detection was 218 nm. Results The linear range of hydrochlorothiazide was 8.07 ~80.74μg/mL(r=0.999 1). The average recovery was 98 ......
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