去卵巢对大鼠膝关节软骨及软骨下骨的影响(1)
【摘 要】目的：观察去卵巢对大鼠膝关节软骨及软骨下骨的影响。方法：将48只3月龄雌性SD大鼠随机分为对照组和去卵巢组(OVX组)，每组24只。对照组不做任何处理，OVX组行双侧卵巢切除术。术后4，8，12周每组随机处死8只，检测血液雌二醇及尿Ⅰ、Ⅱ型胶原C末端肽(CTX-Ⅰ、CTX-Ⅱ)水平，关节软骨行Mankin评分及软骨下骨行Micro-CT检查。结果：与对照组比较，术后4，8，12周OVX组雌二醇水平明显降低，CTX-Ⅰ水平明显增高，差异有统计学意义(P < 0.01)；术后4周2组CTX-Ⅱ水平比较，差异无统计学意义(P > 0.05)；术后8，12周OVX组CTX-Ⅱ水平明显高于对照组，差异有统计学意义(P < 0.01)。术后4，8周2组Mankin评分比较，差异无统计学意义(P > 0.05)；术后12周OVX组Mankin评分较对照组增高，差異有统计学意义(P < 0.01)。术后4，8，12周OVX组骨体积分数、骨小梁厚度较对照组降低，骨小梁分离度较对照组增加，差异有统计学意义(P < 0.01或P < 0.05)；术后4周2组间骨小梁数量比较，差异无统计学意义(P > 0.05)；术后8，12周OVX组骨小梁数量较对照组降低，差异有统计学意义(P < 0.05)。结论：去卵巢使血清雌激素水平减少，从而导致大鼠膝关节软骨退变及软骨下骨骨质疏松。软骨下骨骨质疏松早于关节软骨的退变，其可能是关节软骨退变的始发因素。
【关键词】 骨关节炎；骨质疏松；去卵巢；关节软骨；软骨下骨；大鼠
【ABSTRACT】Objective：To observe the effect of ovariectomy on cartilage and subchondral bone of knee joint of rats.Methods：Forty eight three-month-old SD female rats were randomly divided into a control group and an ovariectomized group(OVX group)，with 24 rats in each group.No treatment was done to the control group ，while the OVX group underwent bilateral ovariectomy.Four，eight and twelve weeks after operation，8 rats of each group were randomly killed to test the levels of blood estradiol，CTX-Ⅰ and CTX-Ⅱ.Mankin score was done to their articular cartilage and microscopic CT was done to their subchondral bone.Results：Compared with the control group，after four，eight and twelve weeks，the levels of estradiol obviously decreased and the levels of significantly increased in the OVX group，and the difference was statistically significant(P < 0.01).Four weeks after operation，the differences of CTX-Ⅱ levels of the two groups were not statistically significant(P > 0.05).Eight and twelve weeks after operation，the level of CTX-Ⅱ in the OVX group was significantly higher than that in the control group，and the difference was statistically significant(P < 0.01).Four and eight weeks after operation，the difference of Mankin score in the two groups was not statistically significant(P > 0.05).Twelve weeks after operation，the Mankin score of the OVX group was higher than that of the control group，and the difference was statistically significant(P < 0.01).Four，eight and twelve weeks after operation，the bone volume fraction and trabecular thickness of the OVX group decreased compared with those in the control group，and the degree of trabecular separation increased，the difference being statistically significant(P < 0.01 or P < 0.05).Four weeks after operation，the difference of the number of trabecular bone in the two groups was not statistically significant(P > 0.05).Eight and twelve weeks after operation，the number of trabecular bone in the OVX group decreased compared with that of the control group，and the difference was statistically significant(P < 0.05).Conclusion：Ovariectomy reduces the level of serum estrogen，resulting in cartilage degeneration and subchondral bone osteoporosis in rats.Subchondral bone osteoporosis is earlier than articular cartilage degeneration，which may be the starting factor of articular cartilage degeneration., 百拇医药(钟培瑞)
【关键词】 骨关节炎；骨质疏松；去卵巢；关节软骨；软骨下骨；大鼠
【ABSTRACT】Objective：To observe the effect of ovariectomy on cartilage and subchondral bone of knee joint of rats.Methods：Forty eight three-month-old SD female rats were randomly divided into a control group and an ovariectomized group(OVX group)，with 24 rats in each group.No treatment was done to the control group ，while the OVX group underwent bilateral ovariectomy.Four，eight and twelve weeks after operation，8 rats of each group were randomly killed to test the levels of blood estradiol，CTX-Ⅰ and CTX-Ⅱ.Mankin score was done to their articular cartilage and microscopic CT was done to their subchondral bone.Results：Compared with the control group，after four，eight and twelve weeks，the levels of estradiol obviously decreased and the levels of significantly increased in the OVX group，and the difference was statistically significant(P < 0.01).Four weeks after operation，the differences of CTX-Ⅱ levels of the two groups were not statistically significant(P > 0.05).Eight and twelve weeks after operation，the level of CTX-Ⅱ in the OVX group was significantly higher than that in the control group，and the difference was statistically significant(P < 0.01).Four and eight weeks after operation，the difference of Mankin score in the two groups was not statistically significant(P > 0.05).Twelve weeks after operation，the Mankin score of the OVX group was higher than that of the control group，and the difference was statistically significant(P < 0.01).Four，eight and twelve weeks after operation，the bone volume fraction and trabecular thickness of the OVX group decreased compared with those in the control group，and the degree of trabecular separation increased，the difference being statistically significant(P < 0.01 or P < 0.05).Four weeks after operation，the difference of the number of trabecular bone in the two groups was not statistically significant(P > 0.05).Eight and twelve weeks after operation，the number of trabecular bone in the OVX group decreased compared with that of the control group，and the difference was statistically significant(P < 0.05).Conclusion：Ovariectomy reduces the level of serum estrogen，resulting in cartilage degeneration and subchondral bone osteoporosis in rats.Subchondral bone osteoporosis is earlier than articular cartilage degeneration，which may be the starting factor of articular cartilage degeneration., 百拇医药(钟培瑞)