陈妍逸 陈鹏

    【摘 要】目的：探讨类风湿关节炎患者中医体质与人类白细胞抗原(HLA)-DRB1基因多态性的关系。方法：根据《中医体质分类与判定》标准设计调查问卷，对200例类风湿关节炎患者进行中医体质类型的调查，以直接碱基序列分析基因分型技术结合特异性引物聚合酶链反应方法测定类风湿关节炎患者的HLA-DRB1基因型，运用统计学方法比较组间体质类型分布差异及组间基因频率的分布差异。结果：阳虚质、气郁质为类风湿关节炎患者易患体质类型;HLA-DRB1*04在不同体质类型患者中分布频率最高，其中阳虚质、气郁质人群HLA-DRB1*04(41.07%、40.00%)基因频率显著高于其他等位基因。结论：中医体质类型与HLA-DRB1基因多态性对类风湿关节炎发生有一定的影响，但其间的相互关系有待进一步研究明確。

    【关键词】 类风湿关节炎;中医体质;HLA-DRB1基因;阳虚质;气郁质

    Investigation on TCM Constitution of Those with Rheumatoid Arthritis and Its Correlation with HLA-DRB1

    Gene Polymorphism

    CHEN Yan-yi，CHEN Peng

    【ABSTRACT】Objective：To explore the relationship between TCM Constitution and HLA-DRB1 gene polymorphism in patients with rheumatoid arthritis.Methods：According to the standards of Classification and Determination of Constitution in TCM，a questionnaire was designed to investigate 200 patients with rheumatoid arthritis.HLA-DRB1 genotypes of patients with rheumatoid arthritis were determined by direct base sequence analysis genotyping technology combined with specific primer polymerase chain reaction ......