董泓 李振彬 王志强 高囡囡 王丽敏 郭栋梁

    【摘 要】目的：探索注射用蜂毒(BVI)对热痛模型小鼠的镇痛作用及对外周血P物质(SP)、

    β-内啡肽(β-EP)水平的影响。方法：选择合格昆明小鼠200只，随机分为BVI大剂量(BVI-H)组、BVI中剂量(BVI-M)组、BVI小剂量(BVI-L)组、吗啡组、模型对照组，给药后分别采用热板法和辐射热法建立小鼠热痛模型，观察不同组别给药后不同时间痛阈的动态变化；实验结束后，心脏取血，分离血清，ELISA法检测血清SP、β-EP水平。结果：BVI各剂量组和吗啡组均显示出明显的镇痛作用，BVI在热板法疼痛模型的作用具有剂量依赖性。BVI对小鼠疼痛模型血清SP、β-EP水平有一定影响，BVI各剂量组和吗啡组均对SP显示出下调效应；血清β-EP水平在BVI-L组升高，BVI-M组和BVI-H组则下降。结论：BVI对小鼠热痛模型具有显著镇痛作用，其相关机制可能与抑制内源性疼痛介质传递、增加内源性镇痛介质分泌等有关。

    【关键词】 疼痛；热痛模型；注射用蜂毒；镇痛；P物质；β-内啡肽；小鼠

    Effects of BVI on Pain Threshold and the Levels of SP and β-EP in Mouse Model with Heat Pain

    DONG Hong，LI Zhen-bin，WANG Zhi-qiang，GAO Nan-nan，WANG Li-min，GUO Dong-liang

    【ABSTRACT】Objective：To explore the analgesic effect of Bee Venom for Injection(BVI)on mouse models with heat pain and its effects on the levels of substance P(SP)and β-Endorphins(EP)in peripheral blood.Methods：Two hundred qualified Kunming mice were selected and randomly divided into the high-dose BVI(BVI-H)group ......