SD大鼠附睾尾部上皮原代细胞不同培养方法的研究
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【摘要】目的：为获得生长状态佳，精子少的附睾上皮原代培养细胞。方法：采取两种方法，取SD大鼠附睾上皮组织，均以酶消化为主，制成细胞悬液，进行培养。结果：方法一：制成的细胞悬液中有较多的精子存在，培养的细胞中成纤维细胞占较大比例；方法二：培养结果比较好，精子少或无，培养的细胞中绝大部分为上皮细胞，且生长速度较快。结论：获得生长状态佳，精子少的附睾上皮原代培养细胞，为研究附睾上皮中与精子功能成熟和受精相关的关键分子功能提供了必要的基础和条件。
【关键词】SD大鼠；附睾上皮；原代细胞培养
Study on primary cultured SD rat epididymal epithelial cell culture methods of different
Zhao Wenzhen He YingHong Wang YunTao Yang YongQin
Department of Histology and Embryology，School of Basic Medicine，Dali University，Dali 671000，China
[Abstract]objective：To obtain good growth state，less sperm epididymal epithelial cells in primary culture.Method：Adopt two kinds of methods.The epididymal epithelial tissues of SD rats were mainly made by enzyme digestion，were made cell suspension and were cultured.Results：Cultured fibroblasts accounted for a large proportion from the first method；The culture results from the second method are good ......
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