张恒岩崔立强王储吴志宏姚杰邱贵兴**

    (1.中国医学科学院 北京协和医学院 北京协和医院骨科及转化医学中心，北京100730；2.北京航空航天大学生物与医学工程学院，北京100191)

    应用microCT对大鼠不对称力脊柱侧凸模型椎体终板微结构的观察*

    张恒岩1崔立强1王储1吴志宏1姚杰2邱贵兴1**

    (1.中国医学科学院 北京协和医学院 北京协和医院骨科及转化医学中心，北京100730；2.北京航空航天大学生物与医学工程学院，北京100191)

    背景：椎体终板由椎体的骨骺骨化形成，脊柱侧凸畸形导致椎体终板承受不正常的应力，此病理情况下椎体终板结构的改变尚未完全明确。

    目的：使用microCT对大鼠不对称力脊柱侧凸模型椎体终板结构的变化进行观察。

    方法：选取5周龄雌性SD大鼠15只，手术将钛钉由大鼠L2、L5右侧横突根部置入椎体，并连结镍钛弹簧建立腰椎不对称力脊柱侧凸模型。7周后使用microCT对脊柱侧凸顶点处椎体终板进行扫描分析，Mimics软件对椎体终板骨性结构及内部孔道结构进行3D重建。

    结果：7周后大鼠脊柱腰段侧凸Cobb角由术后即刻平均25.7°±2.4°进展至平均61.5°±5.4°，microCT平扫重建数据显示脊柱侧凸的凹侧椎体终板较凸侧明显变薄，其内部孔道结构稀疏，骨体积分数(BV/TV)明显增加。

    结论：腰椎不对称力导致脊柱侧凸的凹侧椎体终板趋向形成一致密的薄骨板，这种病理改变可能对凹侧椎体的生长及椎间盘的营养产生不利影响。

    脊柱；脊柱侧凸；模型，动物；大鼠

    Background:The vertebral endplate is formed by epiphyseal ossification of the vertebral body.Abnormal mechanical loading appears inscoliosisascompared to normal spines.Howthe vertebral endplatewaschangedinscoliosisisnotwellunderstood. Objective:To determine structural changes of vertebral endplate in asymmetric load scoliosis rat models by microCT.

    Methods:Fifteen 5-week-old female Sprague-Dawley rats were selected to develop lumbar scoliosis by using a nickel-titanium(NT)coil spring.Two bone screws were implanted in vertebral bodies via the roots of right transverse processes of L2 and L5.The two ends of NT coil spring attached to L2 and L5 screws.MicroCT was used to analyze the vertebral endplate at the curve apex in rat lumbar scoliosis models after 7 weeks and the 3D models of the endplate bone structure and the internal canal structure were reconstructed by Mimics software. ......