骨癌痛大鼠鞘内镇痛对不同组织MOR表达水平研究(1)
【摘 要】目的：探索在骨癌痛SD大鼠模型中，比較应用阿片类药物吗啡通过外周给药及鞘内留置管给药时，镇痛效果及不同组织MOR表达水平的差异。方法：雌性SD大鼠(150-180g)24只，全部大鼠于股骨注射Walker256大鼠腹水癌细胞制作疼痛模型。癌痛造模成功后，完全随机法分为空白组、腹腔组和鞘内组，鞘内组经L6-S1棘突间隙放置鞘内导管。空白组注射生理盐水，腹腔(1ml，1次/d)、鞘内(20ul，1次/d)；腹腔组腹腔注射吗啡(1.25mg/1ml，1次/d)，鞘内注射生理盐水(20ul，1次/d)；鞘内组腹腔注射生理盐水(1ml，1次/d)，鞘内注射等效腹腔组镇痛效应的吗啡(0.025mg/20ul，1次/d)，连续镇痛16d。观察大鼠痛阈变化及脊髓组织和肿瘤组织MOR表达水平。结果：镇痛后腹腔组和鞘内组比较，痛阈相近。腹腔组和鞘内组分别与空白组比较，痛阈均显著增加。在骨肿瘤组织MOR表达方面：空白组、鞘内组和腹腔组MOR/GAPDH蛋白灰度比值分别为0.33±0.07、0.32±0.07、0.50±0.11 ，鞘内组和空白组分别明显低于腹腔组(P<0.05)，鞘内组和空白组无统计学差异。在脊髓组织MOR表达方面：空白组、鞘内组和腹腔组MOR/GAPDH蛋白灰度比值分别为0.65±0.09、0.64±0.10、0.62±0.12，三组间无统计学差异。结论：鞘内给与阿片类药物可以达到良好镇痛效果。鞘内和全身应用阿片类药物对脊髓组织MOR表达无影响，全身应用阿片类药物促进肿瘤组织MOR表达，而鞘内应用阿片类药物对肿瘤组织MOR表达无影响。
【关键词】鞘内镇痛；?阿片受体；骨癌痛
【Abstract】Objective：To comPare the exPression level of MOR in different tissue and analgesic result with morPhine， through intraPertoneal and intrathecal in the bone cancer Pain model rats.Methods： 24 female SPrague-Dawley rats (150-180 g) were injected Walker256 carcinoma cells into the femur to Produce a bone cancer Pain model. after cancer Pain model was successfully established， they were randomly divided into control grouP， intraPeritoneal grouP and intrathecal grouP. The intrathecal grouP was Placed intrathecal catheter in L6-S1 sPinal sPine sPace. The control grouP was intraPeritoneally injected with saline (1 ml， q.d.)， intrathecal saline (20 μl， q.d.)； the intraPeritoneal grouP was intraPeritoneally injected with morPhine (1.25 mg/1 ml， q.d.)， intrathecal injection of Saline (20ul， q.d.)； Intrathecal grouP was injected intraPeritoneal saline (1ml， q.d.)， intrathecal morPhine (0.025mg/20ul， q.q.) which has an equivalent dose with the intraPeritoneal grouP， continuous analgesia for 16 days. Observed changes in Pain threshold and sPinal cord and tumor tissue MOR exPression levels.Results： After analgesia， comPared with the intraPeritoneal grouP， the analgesic effect of the intrathecal grouP was similar. The Pain threshold was significantly increased in the intraPeritoneal grouP and the intrathecal grouP， comPared with control grouP. MOR exPression in tumor tissues： gray ratios of MOR/GAPDH in control grouP， intrathecal grouP， and intraPeritoneal grouP were resPectively 0.33±0.07， 0.32±0.07，0.50±0.11， MOR exPression were significantly lower in the intrathecal grouP and control grouP than in the intraPeritoneal grouP (P <0.05)， and there was no statistical difference between the intrathecal grouP and control grouP. MOR exPression in sPinal cord： gray ratios of MOR/GAPDH in control grouP， intrathecal grouP， and intraPeritoneal grouP were resPectively 0.65±0.09， 0.64±0.10， and 0.62±0.12. There was no statistical difference between the three grouPs. Conclusion： Intrathecal administration of oPioids can achieve good analgesia. Intrathecal and intraPeritoneal aPPlication of oPioids had no effect on the exPression of MOR in sPinal cord tissues. IntraPeritoneal aPPlication of oPioids Promoted the exPression of MOR in tumor tissues， and intrathecal aPPlication of oPioids had little effect on the exPression of MOR in tumor tissues., 百拇医药(刘赫琪?刘伟?岳文涛?王子宇?江妹?罗太君)
【关键词】鞘内镇痛；?阿片受体；骨癌痛
【Abstract】Objective：To comPare the exPression level of MOR in different tissue and analgesic result with morPhine， through intraPertoneal and intrathecal in the bone cancer Pain model rats.Methods： 24 female SPrague-Dawley rats (150-180 g) were injected Walker256 carcinoma cells into the femur to Produce a bone cancer Pain model. after cancer Pain model was successfully established， they were randomly divided into control grouP， intraPeritoneal grouP and intrathecal grouP. The intrathecal grouP was Placed intrathecal catheter in L6-S1 sPinal sPine sPace. The control grouP was intraPeritoneally injected with saline (1 ml， q.d.)， intrathecal saline (20 μl， q.d.)； the intraPeritoneal grouP was intraPeritoneally injected with morPhine (1.25 mg/1 ml， q.d.)， intrathecal injection of Saline (20ul， q.d.)； Intrathecal grouP was injected intraPeritoneal saline (1ml， q.d.)， intrathecal morPhine (0.025mg/20ul， q.q.) which has an equivalent dose with the intraPeritoneal grouP， continuous analgesia for 16 days. Observed changes in Pain threshold and sPinal cord and tumor tissue MOR exPression levels.Results： After analgesia， comPared with the intraPeritoneal grouP， the analgesic effect of the intrathecal grouP was similar. The Pain threshold was significantly increased in the intraPeritoneal grouP and the intrathecal grouP， comPared with control grouP. MOR exPression in tumor tissues： gray ratios of MOR/GAPDH in control grouP， intrathecal grouP， and intraPeritoneal grouP were resPectively 0.33±0.07， 0.32±0.07，0.50±0.11， MOR exPression were significantly lower in the intrathecal grouP and control grouP than in the intraPeritoneal grouP (P <0.05)， and there was no statistical difference between the intrathecal grouP and control grouP. MOR exPression in sPinal cord： gray ratios of MOR/GAPDH in control grouP， intrathecal grouP， and intraPeritoneal grouP were resPectively 0.65±0.09， 0.64±0.10， and 0.62±0.12. There was no statistical difference between the three grouPs. Conclusion： Intrathecal administration of oPioids can achieve good analgesia. Intrathecal and intraPeritoneal aPPlication of oPioids had no effect on the exPression of MOR in sPinal cord tissues. IntraPeritoneal aPPlication of oPioids Promoted the exPression of MOR in tumor tissues， and intrathecal aPPlication of oPioids had little effect on the exPression of MOR in tumor tissues., 百拇医药(刘赫琪?刘伟?岳文涛?王子宇?江妹?罗太君)