[摘要]目的探究胰高血糖素样肽-1(GLP-1)受体激动剂Exendin-4及内源性GLP-1对海马CA3区神经元自发放电的调控作用。方法利用在体细胞外单细胞电生理记录方法，观察三管微电极微压力注射10 μmol/L Exendin-4和10 μmol/L Exendin-9-39(GLP-1受体阻断剂)对大鼠海马CA3区神经元自发放电频率的影响。结果在记录到的22个海马CA3区神经元中，Exendin-4使17个神经元放电频率显著增高(t=6.286，Plt;0.01)，平均升高(149.67±18.94)%，与生理盐水组相比差异有显著性(Z=3.571，Plt;0.01)。在记录到的14个海马CA3区神经元中，Exendin-9-39使10个神经元放电频率显著降低(t=7.968，Plt;0.01)，平均降低(61.90±6.10)%，与生理盐水组相比差异有显著性(Z=3.145，Plt;0.01)。结论Exendin-4兴奋海马CA3区神经元，内源性GLP-1参与调节海马CA3区神经元自发放电。

    [关键词]胰高血糖素样肽1；艾塞那肽；CA3区，海马；电生理学；大鼠

    [中图分类号]R338.2[文献标志码]A[文章编号]2096-5532(2023)03-0353-04

    doi：10.11712/jms.2096-5532.2023.59.074[开放科学(资源服务)标识码(OSID)]

    [网络出版]https：//kns.cnki.net/kcms2/detail/37.1517.R.20230719.1612.002.html;2023-07-2014：53：18

    ROLE OF EXENDIN-4 AND ENDOGENOUS GLUCAGON-LIKE PEPTIDE-1 IN REGULATING THE SPONTANEOUS DISCHARGE OF NEURONS IN THE HIPPOCAMPAL CA3 REGION OF RATS SUN Huizhe， LIU Cui， SHEN Fangshuai， CHEN Xinyi， XUE Yan， CHEN Lei (Department of Physiology and Pathophysiology， School of Basic Medicine， Qingdao University Medical College， Qingdao 266071， China)

    [ABSTRACT]ObjectiveTo investigate the role of the glucagon-like peptide 1 (GLP-1) receptor agonist Exendin-4 and endogenous GLP-1 in regulating spontaneous discharge of neurons in the hippocampal CA3 region. MethodsIn vivo extracellular single-unit recordings were used to observe the effect of three-barrel glass micro-electrode micro-pressure injection of 10 μmol/L Exendin-4 versus 10 μmol/L Exendin-9-39 (a GLP-1 receptor antagonist) on the spontaneous discharge frequency of neurons in the hippocampal CA3 region. ResultsAmong the 22 neurons recorded in the hippocampal CA3 region ......