[关键词] 羟多巴胺；铁蛋白质类；小神经胶质细胞；帕金森病[中图分类号]R742.5；R341.3 [文献标志码]A [文章编号] 2096-5532(2025)03-0359-04doi：10.11712/jms.2096-5532.2025.61.078 [开放科学(资源服务)标识码(OSID)][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250709.1559.005;

    2025-07-10 11：55：34

    Efect of 6-hydroxydopamineon the expressonofferitin inBV2 microglialcellsJING Cuiting，LIXiaoqian，QU Yan，XIE Junxia，WANG Jun(Departmentof Physiology，Scholof Basic Medicine，Qingdao University MedicalCollge，Qingdao 266071，China)

    [Abstract]ObjetiveToinvestigate theeffctof6-hydroxydopamine(6-OHDA)ontheexpressionofferritin inBV2 microglialcell.MetodsAfterBV2microglialcelswere treated withdiffrentconentrationsof6-OHDA(1o，25，5Oμmol/L) for24hours，theamountoflactatedehydrogenase(DH)released was measured toreflectcellviability，andWesternblotwas usedtomeasurethechanges inthe protein expressionlevelsofferitin heavychain1(FTH1)andferrtinlightchain(FTL)in cells.ResultsCompared with thecontrol group without6-OHDA treatment，the BV2 microglial cels treated with 10μmol/L 6-OHDA for 24 hours had no significant change in the amount of LDH released，and the groups treated with 25 and 50μmol/L 6- OHDA and the maximum enzyme activity control group had a significant increase in the amount of LDH released ?F=92.750 ......