Xu Qin

    (Department of Clinical Laboratory Science,Zunyi Meidcal College,Guizhou Zunyi 563003, China)

    Determination of Barbiturates in urine by GC/MS

    Xu Qin

    (Department of Clinical Laboratory Science,Zunyi Meidcal College,Guizhou Zunyi 563003, China)

    ObjectiveTo introduce a highly selective and sensitive procedure for isolating and identifying barbiturates in human urin established by University of kentucky,USA.Methods①An aliquot of urine is extracted with a Toxi-tube and derivatized with N,N-dimethylformamide dimethyl acetal(DMF-DMA).②The sample is analyzed by GC/MS and selected ion monitoring mode (SIM)was used for identification Drug Quant software was used for quantification.for the following barbiturates:butalbital,amobarbital,pentobarbital,secobarbital and phenobarbital in human urin.Results①The cutoff level for GC/MS is 100 ng/ml for amobarbital,butalbital,pentobarbital,phenobarbital and 30 ng/mL for secobarbital.②The method/procedure also showed high between run and within run precision and accuracy.ConlusionsThis method /procedure is more selective ......