Ephrin B2 expression in Kaposi sarcoma is induced by human herpesvirus type 8: phenotype switch from venous to arterial endothelium
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《血液学杂志》
the Departments of Medicine and Pathology, University of Southern California Keck School of Medicine, Los Angeles, CA.
Abstract
Kaposi sarcoma (KS) is an angioproliferative tumor derived from endothelial cells in which tumor cells form aberrant vascular structures. Ephrin B2 and ephrin B4 (EphB4) are artery- and vein-specific proteins, respectively, with critical roles in vessel maturation. We investigated whether the disorganized KS vasculature was due to unbalanced expression of ephrin B2 and EphB4. Secondly, we wished to determine if human herpesvirus type 8 (HHV-8), the viral agent associated with KS, regulates ephrin B2 and EphB4. An arterial phenotype was observed in KS tissue and cell lines, as shown by abundant expression of ephrin B2 with little or no EphB4. Infection of venous endothelial cells with HHV-8 resulted in a phenotype switch from EphB4 to ephrin B2, similar to that seen with vascular endothelial growth factor (VEGF). The HHV-8 effect on ephrin B2 expression was reproduced with the HHV-8-specific viral G-protein-coupled receptor. We also showed that ephrin B2 expression is required for KS cell viability by knock down with siRNA. KS is the first example of a human tumor with a predominantly arterial phenotype. This predominance can be attributed to expression of HHV-8 proteins and their downstream effects. Ephrin B2 is thus an important novel factor in KS biology and a potential target for therapy.
Introduction
Kaposi sarcoma (KS) manifests as a multifocal angioproliferative disease, most commonly of the skin and mucus membranes with subsequent spread to visceral organs.1 Hallmarks of the disease are angiogenesis, edema, infiltration of lymphomononuclear cells, and growth of spindle-shaped tumor cells. Pathologically, established lesions exhibit an extensive vascular network of slitlike spaces. Abnormal spindle-shaped endothelial cells (tumor cells) line the tumor vessels, which also lack basement membranes. Defective vasculature results in an accumulation of the blood components including albumin and red and mononuclear cells in the lesions.1 The KS tumor is endothelial in origin; the tumor cells express many endothelial markers, including lectin binding sites for Ulex europeaus agglutinin-1 (UEA-1), CD34, EN-4, pathologische anatomie Leiden endothelium (PAL-E),2 and the endothelial cell-specific tyrosine kinase receptors vascular endothelial growth factor receptor 1 (VEGFR-1; fetal liver tyrosine kinase 1 [Flt-1]), VEGFR-2 (fetal liver kinase-1/kinase domain receptor [Flk-1/KDR]), VEGFR-3 (Flt-4), Tie-1, and Tie-2 (Masood et al3; R.M. and P.S.G., unpublished data, 2004). KS cells coexpress lymphatic endothelial cell-related proteins including lymphatic vessel endothelial hyaluronan receptor (LYVE) and podoplanin.4
The gammaherpesvirus human herpesvirus type 8 (HHV-8) is considered the etiologic agent for the disease. In 1994 sequences of this herpes virus were identified in KS tumor tissue,5 and subsequent molecular epidemiology studies have shown that nearly all KS tumors contain viral genome. Seroepidemiology studies show that HIV-infected patients with KS have the highest prevalence of HHV-8 and secondly that those with HIV infection but no KS have increased risk of development of KS over the ensuing years if they are also seropositive for HHV-8.6 Direct evidence for the role of HHV-8 in KS is the transformation of bone marrow endothelial cells after infection with HHV-8.7 A number of HHV-8-encoded genes could contribute to cellular transformation (reviewed in Mihalcea et al8). However, the most evidence has accumulated for the viral G-protein-coupled receptor (vGPCR) in this role.9
Vascular endothelium is functionally and phenotypically divided into 2 distinct populations. Venous endothelial cells express the tyrosine kinase receptor EphB4, whereas arterial endothelial cells express its membrane-bound ligand ephrin B2.10-12 Cell-cell contact is required for binding and activation of Eph's by clustered membrane-attached ephrins.13 Full activation of Eph dimers is accomplished by autophosphorylation of juxtamembrane tyrosine residues.14,15 In addition to forward signaling through the Eph's, reverse signaling can occur through phosphorylation of the ephrin Bs.16-18
Targeted gene knockouts of either ephrin B2 or EphB4 in mice show similar phenotypes with disrupted vessel maturation and early embryonic lethality.11,12 Artery and vein phenotypes are determined very early in development, at the time of mesoderm differentiation to the vascular lineage.19 The ephrin B2/EphB4 ligand/receptor pair is important in vasculogenesis in the embryo and is also essential for defining the boundaries between arterial and venous domains10 that persist in the adult.20 It has been shown in vitro that EphB4-mediated forward signaling restricts intermingling of cells and supports cellular segregation, whereas reverse signaling from ephrin B2 stimulates migration and sprouting angiogenesis.21,22
The current model for artery/vein differentiation is that venous differentiation is the default pathway. VEGF appears to be a permissive signal for attaining the arterial phenotype through up-regulation of the Notch receptor and its ligand delta-like 4 (Dll-4) in arterial endothelial cells.23,24
We investigated whether KS tumor cells are derived from arterial or venous endothelium. In addition, we investigated whether HHV-8 has an effect on expression of arterial or venous markers in a model of KS. KS tumor cells were found to express the ephrin B2 arterial marker. Further, ephrin B2 expression was induced by HHV-8 and also by the HHV-8-specific vGPCR in KS and endothelial cell lines. Ephrin B2 is a potential target for treatment of KS because inhibition of ephrin B2 expression or signaling was detrimental to KS cell viability and function.
Patients, materials, and methods
Cell lines and reagents
Human umbilical vascular endothelial cells (HUVECs) were from Clonetics (San Diego, CA) and were maintained in endothelial growth medium 2 (EGM-2; Clonetics). EGM-2 is supplied as endothelial basal medium 2 (EBM-2) with supplements for fresh addition: fetal calf serum (FCS) to 2%, human epidermal growth factor (hEGF), hydrocortisone, VEGF, basic fibroblast growth factor (bFGF), recombinant insulin-like growth factor 1 (IGF-1), ascorbic acid, heparin, gentamicin, and Amphotericin-B. T1 human fibroblast line was from Dr Peter Jones (University of Southern California [USC]). BC-1 (HHV-8-positive, Epstein-Barr virus [EBV]-positive) and BC-3 (HHV-8-positive, EBV-negative) human pleural effusion lymphoma cell lines25 and monoclonal antibodies to latency-associated nuclear antigen 1 (LANA1) and HHV-8 lytic-phase ORF59 protein (ORF59) were the kind gift of Dr Dharam Ablashi (Advanced Biotechnologies, Columbia, MD). The KS-SLK cell line was isolated from a patient with classic KS.26 SCC-25 and SCC-15 cell lines were from the American Type Culture Collection (ATCC; Manassas VA). Antibodies to EphB4, ephrin B2, CD148, platelet endothelial cell adhesion molecule 1 (PECAM-1; all polyclonal), and VEGFR-2 (monoclonal) were from Santa Cruz Biotechnology (Santa Cruz, CA). VEGFR-3/Fc and monoclonal antibodies to human VEGF, VEGF-C, interleukin-6 (IL-6), IL-8, and oncostatin-M were from R&D Systems (Minneapolis, MN). Antibodies to p44/42 mitogen-activated protein kinase (MAPK; polyclonal) and phospho-p44/42 MAPK (monoclonal) were from New England Biolabs (Beverly, MA), and antiphosphotyrosine monoclonal antibody was from Upstate (Charlotte, VA). Expression vectors pCEFL-KS-associated herpesvirus-GPCR (pCEFL-KSHV-GPCR) and pCEFL were the kind gift of Dr Enrique Mesri (Cornell University, NewYork, NY). Expression vectors for HHV-8 LANAand LANA440, which lacks the nuclear localization domain, were kindly provided by Dr Matthew Rettig, Veteran's Administration Greater LosAngeles Healthcare System.
Collection and preparation of human tissue
Human cutaneous KS biopsies were obtained under local anesthesia with informed consent from patients at the Los Angeles County (LAC)/USC Medical Center, using an institutional review board (IRB)-approved consent form. Biopsies were processed for either total RNA, paraffin blocks, or frozen tissue blocks in optimum cutting temperature (OCT). Total RNA was extracted by homogenization in RNAzol (Tel-Test, Friendswood, TX). cDNAs were synthesized by reverse transcriptase using a random hexamer primer (Superscript II; Invitrogen, Carlsbad, CA).
Preparation of digoxigenin-labeled RNA probes and in situ hybridization
Ephrin B2 and EphB4 polymerase chain reaction (PCR) products from the primers shown in Table 1 for in situ hybridization (ISH) were cloned into pGEM-T Easy Vector System (Promega, Madison, WI). DNA sequencing confirmed the orientation and authenticity of the inserts. Antisense or sense digoxigenin (DIG)-labeled RNA probes were created by run-off transcription from T7 or SP6 promoters using a DIG RNA labeling kit (Roche, Indianapolis, IN). RNA probes were quantitated by spot assay according to the manufacturer's instructions.
Frozen sections were subject to ISH essentially as previously described,27 except that the hybridization step was overnight at 42°C with 25 ng antisense or sense RNAprobes. Hybridization signal was detected using alkaline-phosphatase-conjugated anti-DIG antibodies (Roche) according to the manufacturer's instructions. Cells were visualized by counterstaining of nucleic acids with Nuclear Fast Red (Vector Laboratories, Burlingame, CA).
Coculture of HUVECs and BC-1
HUVECs were grown to 50% to 70% confluence in EGM-2 on gelatin-coated chamber slides (Nalge Nunc International, Naperville, IL). Coculture with BC-1 or BC-3 was essentially as described by Sakurada et al.28 Briefly, BC-1 or BC-3 cells were pretreated with TPA (12-O-tetradecanoyl-phorbol 13-acetate; 20 ng/mL) to induce virus for 48 hours and then added to the HUVEC culture at a ratio of 10:1 for cocultivation for 2 days. The HUVECs were washed extensively with phosphate-buffered saline (PBS) to remove the attached BC-1 or BC-3 cells.
Preparation of cDNA and RT-PCR
Cell viability assay
KS-SLK cells were seeded at a density of 1 x 104 per well in 48-well plates on day 0 in appropriate growth medium containing 2% FCS. On the following day, the medium was changed and cells were transfected with 0, 10, or 100 nM siRNA. Forty-eight hours after transfection, viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as previously described.29 A similar procedure was followed for studies with neutralizing antibody and VEGFR-3/Fc. The following day, plating medium was replaced with 100 ng/mL antibody or recombinant Fc fusion protein and viability assessed 48 hours later with MTT.
Immunofluorescence studies
Cells cultured on chamber slides or frozen sections of KS biopsy material were fixed in 4% paraformaldehyde and preincubated with blocking buffer (0.2% Triton-X100, 1% bovine serum albumin [BSA] in PBS) for 20 minutes, followed by incubation with antibodies to EphB4, ephrin B2, CD148, LANA1 or ORF59 (1:100 dilution in PBS) in blocking buffer at 4°C for 16 hours. Slides were then incubated with the appropriate fluorescein- or rhodamine-conjugated secondary antibodies (Sigma-Aldrich, St Louis, MO). Nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole dihydrochloride hydrate). Images were obtained using a Olympus AX70 fluorescence microscope and Spot v2.2.2 (Diagnostic Instruments, Sterling Heights, MI) digital imaging system.
Immunofluorescence detection of ephrin B2 with EphB4-Fc
Frozen sections fixed in 4% paraformaldehyde and blocked with 20% FCS were incubated with 5 μg/mL EphB4/Fc (R&D Systems) for 1 hour at room temperature. Sections were then incubated with 10 μg/mL rabbit anti-human immunoglobulin G-fluorescein isothiocyanate (IgG-FITC) in PBS (Jackson ImmunoResearch Laboratories, West Grove, PA) at room temperature for 1 hour. Nuclei were counterstained with DAPI and sections mounted. Human IgG Fc (Jackson ImmunoResearch Laboratories) was used as the negative control.
Western blot
Crude cell lysates were prepared, quantitated, fractionated, and transferred to membranes as described previously.29 Membranes were blocked with 5% nonfat milk prior to incubation with antibody to ephrin B2 (1:5000 dilution) at 4°C for 16 hours. Secondary antibody (1:5000 dilution) conjugated with horseradish peroxidase was applied for 1 hour at 25°C. The membranes were developed using the SuperSignal West Femto Maximum sensitivity chemiluminescent substrate (Pierce, Rockford, IL) according to the manufacturer's instructions. Membranes were stripped using Restore Western Blot Stripping Buffer (Pierce) and reprobed with EphB4 or -actin.
Synthesis of ephrin B2 and EphB4 siRNA by in vitro transcription
Ephrin B2 or EphB4 knockdown with siRNA in HUVECs grown with or without VEGF
Duplicate cultures of HUVECs were seeded on 8-well chamber slides coated with fibronectin and grown overnight in EGM-2. Sixteen hours later, medium was replaced with basal medium (EBM-2) supplemented with 5% fetal calf serum (FCS) and EGM-2 BulletKit supplements bFGF, hEGF, and recombinant insulin-like growth factor 1 (R3-IGF-1) at the concentrations provided by the manufacturer in half of the wells or 5% FCS and 10 ng/mL recombinant human VEGF (rhVEGF; R&D Systems) in the remaining wells. After 2 hours incubation at 37°C, all cells were transfected using Lipofectamine 2000 (1 μg/mL; Invitrogen) and 10 nM specific siRNAs in Opti-MEM-1 serum-free medium (Invitrogen). Following transfection for 2 hours in Opti-MEM-1, EBM-2 medium containing growth supplements was replaced in the appropriate wells. After 48 hours, the cells were stained with crystal violet and immediately photographed at x 10 magnification.
Construction of ephrin B2 reporter plasmids
Human ephrin B2 5'-flanking DNA from -2491 to -11 with respect to the translation start site was amplified from BACPAC clone RP11-297I6 (BacPac Resources, Children's Hospital, Oakland, CA) using theAdvantage GC Genomic PCR kit (Clontech) to overcome the large tracts of GC-rich sequence in the target area. Primers were designed to contain MluI sites for cloning into pGL3Basic Vector (Promega). Orientation of the resulting clones was confirmed by restriction digest analysis. The correct clone was designated pEFNB2-2491/-11luc. Digestion of this clone with either KpnIor SacI followed by recircularization yielded pEFNB2-1242/-11luc and pEFNB2-577/-11luc, respectively. Plasmid DNAs used for transient transfections were purified using a Mega Prep kit (QIAGEN, Valencia, CA).
Transient transfection and reporter assays
HUVECs (0.8 x 104 cells/well in 24-well plates) maintained in EGM-2 media were transiently cotransfected with 0.5 μg/well ephrin B2 promoter-luciferase constructs together with 50 ng/well either pCEFL or pCEFL-KSHV-GPCR, using Superfect reagent (QIAGEN) according to the manufacturer's instructions. Cells were harvested 48 hours after transfection and lysed with Luciferase cell lysis buffer (Promega). Luciferase activity was assayed using the Luciferase Assay System (Promega) according to the manufacturer's instructions. Luciferase was normalized to protein because pCEFL-vGPCR induced the expression of -galactosidase from pCMV-Sport-gal (Invitrogen).
Apoptosis enzyme-linked immunosorbent assay (ELISA) and caspase activation assays
Apoptosis was detected using the Cell Death Detection ELISAPlus Kit (Roche) following the manufacturer's instructions 48 hours after siRNA transfection of 2500 KS-SLK cells/well. For caspase activation assays, 2.5 x 106 cells were transfected with siRNAs. Forty-eight hours after transfection, activation of caspases 8 and 9 were determined on cytosolic extracts using the caspase 8 and caspase 9 colorimetric assays (R&D Systems) according to the manufacturer's instructions.
Results
Ephrin B2 but not EphB4 transcripts in KS tumors
The highly vascular nature of KS lesions and the probable endothelial cell origin of the tumor cells prompted investigation of expression of EphB4 and ephrin B2, which are markers for venous and arterial endothelial cells, respectively. Ephrin B2 but not EphB4 transcripts were detected in tumor cells of KS biopsies by in situ hybridization (Figure 1A). Comparison of the positive signal with ephrin B2 antisense probe and tumor cells as shown by hematoxylin and eosin (H&E) staining shows that ephrin B2 expression is limited to the areas of the biopsy that contain tumor cells. Specificity of the EphB4 antisense probe was confirmed by positive signal for transcripts in head and neck squamous cell carcinoma (HNSCC), which we have shown expresses EphB4.30
EphB4 binding sites in KS tumors
Additional evidence for the expression of ephrin B2 in KS tumor tissue is afforded by the localization of EphB4/Fc signal to tumor cells detected by FITC-conjugated anti-human Fc antibody. Because ephrin B2 is the only ligand for EphB4, this reagent is specific for the expression of ephrin B2 (Figure 1Bi). An adjacent section treated only with human Fc and the secondary reagents shows no specific signal.
Coexpression of ephrin B2 and HHV-8 proteins in KS tumors
In other studies, 2-color confocal microscopy demonstrated the presence of the HHV-8 latency protein LANA1 in the ephrin B2-positive cells (Figure 1Ci), indicating that it is the tumor cells, not tumor vessels, that are expressing this arterial marker. Staining of tumor biopsy with the vessel-specific PECAM-1/CD31 antibody revealed the highly vascular nature of this tumor (Figure 1Ciii).
Ephrin B2 but not EphB4 is present in KS biopsies and cell lines
Western blots on KS biopsy lysates confirmed the expression of ephrin B2 but not EphB4. Matched normal skin was obtained for 2 of the cases and these expressed neither ephrin B2 nor EphB4 (Figure 1D). In addition, the immortalized KS cell line KS-SLK and a primary isolate, KS 6-3, showed the same pattern of expression as the biopsy material. The EphB4 antibody could detect protein, as shown by signal in squamous cell carcinomas SCC-15 and SCC-25.
Arterial phenotype switch in venous endothelial cells infected with HHV-8
We next asked whether HHV-8, the presumed etiologic agent for KS, itself induced expression of ephrin B2 and repressed EphB4 expression in endothelial cells. Coculture of HUVECs and BC-1 lymphoma cells, which are productively infected with HHV-8, results in effective infection of the endothelial cells.28 The attached monolayers of endothelial cells remaining after extensive washing were examined for ephrin B2 and EphB4 by RT-PCR and immunofluorescence. HUVECs express EphB4 venous marker strongly at the RNA level but not ephrin B2 (Figure 2A). In contrast, HHV-8-infected cultures (HUVEC/BC-1 and HUVEC/BC-3) express ephrin B2, whereas EphB4 transcripts are almost absent. Ephrin B2 signal in infected cell cultures is not due to residual BC-1 cells, as they do not express ephrin B2 and only express low levels of EphB4 mRNA (Figure 2A). No evidence of ephrin B2 and EphB4 protein in BC-1 and BC-3 cell lines was found by Western blot (Figure 2B). Positive controls for the antibodies run on the same gel are shown in Figure 1D (SCC-15).
Coexpression of arterial markers and HHV-8 proteins in infected endothelial cells
Immunofluorescence analysis of cultures of HUVECs and HUVEC/HHV-8 for artery/vein markers and viral proteins was undertaken to determine whether changes in protein expression mirrored that seen in the RNA. In addition, cellular localization of the proteins could be determined. Consistent with the RT-PCR data, HUVECs are ephrin B2 negative and EphB4 positive (Figure 2Ci,xiii). As expected, they do not express any HHV-8 LANA1 (Figure 2Cii,xiv). Coculture of BC-1 cells, which are productively infected with HHV-8, resulted in infection of HUVECs as shown by presence of viral proteins LANA1 and ORF59 (Figure 2Cvi,xviii). HHV-8-infected HUVECs now express ephrin B2 but not EphB4 (Figure 2Cv,xvii,xxi, respectively). Expression of ephrin B2 and LANA1 cocluster is shown by yellow signal in the merged image (Figure 2Cviii). HHV-8-infected HUVECs positive for ephrin B2 and negative for EphB4 also express the arterial marker CD14819 (Figure 2Cx,xxii). Expression of ephrin B2 and CD148 cocluster is shown by yellow signal in the merged image (Figure 2Cxii). Uninfected HUVECs expressing EphB4 were negative for CD148 (not shown).
HHV-8 vGPCR induces ephrin B2 protein
To test whether individual viral proteins could induce the expression of ephrin B2 seen with the whole virus, KS-SLK cells were stably transfected with HHV-8 LANA, LANA440 (which lacks the nuclear localization domain), or vGPCR. Western blot of stable clones showed overexpression of ephrin B2 in KS-SLK cells transfected with vGPCR compared with LANA, LANA440, or vector alone (pCEFL; Figure 3A). Expression of HHV-8 proteins in stable clones was confirmed by RT-PCR (Figure 3B). Transient transfection of KS-SLK cells with vGPCR resulted in induction of ephrin B2 expression as detected by immunofluorescence (Figure 3C) compared with vector alone (pCEFL). EphB4 was not induced in KS-SLK-vGPCR compared with KS-SLK-pCEFL, and negligible EphB4 expression in KS-SLK cells (data not shown) precluded determination of whether it was repressed in the KS-SLK-vGPCR transfectants.
Transcriptional regulation of ephrin B2 by HHV-8 vGPCR
Since we had shown that HHV-8 induced expression of ephrin B2 in HUVECs, we next asked if this could be mediated by a transcriptional effect. Ephrin B2 5'-flanking DNA-luciferase reporter plasmids were constructed as described in "Patients, materials, and methods" and transiently transfected into HUVECs. Ephrin B2 5'-flanking DNA sequences -2491/-11 have minimal activity in HUVECs, consistent with ephrin B2 being an arterial not venous marker. However, we have noted that HUVECs in culture do express some ephrin B2 RNA. Cotransfection of HHV-8 vGPCR induces ephrin B2 transcription approximately 10-fold above background compared with the control expression vector pCEFL (Figure 3D). Roughly equal induction was seen with ephrin B2 sequences -2491/-11, -1242/-11, or -577/-11, which indicates that elements between -577 and -11 are sufficient to mediate the response to vGPCR.
Expression of ephrin B2 is regulated by KS growth factors
We next asked whether known KS growth factors could be involved in the vGPCR-mediated induction of ephrin B2 expression. KS-SLK-vGPCR stable transfectants were treated with neutralizing antibodies to oncostatin-M, IL-6, IL-8, VEGF, or VEGF-C for 36 hours. Neutralization of VEGF almost completely blocked expression of ephrin B2 in KS-SLK-vGPCR cells (Figure 4A). A lesser but significant decrease in ephrin B2 was seen with the IL-8, IL-6, and VEGF-C antibody treatments. No appreciable effect was seen with neutralization of oncostatin-M. The biochemical activity of the VEGF neutralizing antibody in these cells was verified by inhibition of VEGFR-2 and p44/42 MAPK phosphorylation (Figure 4B). The biologic activity of antibody treatment was illustrated by decreased cell viability in SLK cells but not in unrelated T1 fibroblasts or squamous cell carcinoma SCC-25 (Figure 4C). A soluble form of the VEGF-C receptor, VEGFR-3/Fc, was used to interfere with VEGF-C stimulation of the cells in lieu of the antibody, which is not neutralizing. VEGF, VEGF-C, IL-8, and IL-6 induced expression of ephrin B2 in HUVECs, whereas EGF and oncostatin-M did not (Figure 4D), thus providing additional evidence that these KS growth factors are integral to the induction of ephrin B2.
Ephrin B2 is necessary for KS and EC viability
Three ephrin B2 siRNAs were synthesized and transfected into KS-SLK cells. Forty-eight-hour treatment with ephrin B2 siRNAs 137 or 254 knocked down ephrin B2 protein by about 70% and inhibited KS-SLK cell viability, whereas ephrin B2 siRNA 63, EphB4 siRNA 50, or scrambled GFP siRNA had little or no effect (Figure 5A-B). Additionally, apoptosis was increased with respect to both mock-treated (Lipofectamine 2000 alone) and control siRNAs (GFPsi and EphB4 si50) with the 2 active ephrin B2 siRNAs (si137 and si254) in KS-SLK and 2 early passage KS cell isolates to a similar degree (Figure 5C). The mechanism of apoptosis was determined to be via the extrinsic or death-receptor-mediated pathway as shown by increase in activation of caspase 8. Under the same conditions, caspase 9 (mitochondrial or intrinsic pathway) was not activated (Figure 5D). KS-SLK cells express high levels of ephrin B2 and these results show that maintenance of ephrin B2 expression is integral to cell viability in this setting.
HUVECs do not express ephrin B2 (Figure 2A), except when stimulated by VEGF as shown in Figure 4B. Ephrin B2 siRNA 254 knocked down expression of ephrin B2 in VEGF-stimulated HUVECs (Figure 5E) and dramatically reduced the number of HUVECs cultured with VEGF as the sole growth factor (Figure 5F). In contrast, no appreciable effect was seen when HUVECs were cultured with a combination of IGF, EGF, and bFGF. As controls, EphB4 siRNA 50 and GFP siRNA had no detrimental effect on HUVECs in either culture condition.
Discussion
KS is a vascular proliferative disorder with tumor cells derived from endothelial cells. Recent identification of phenotypic markers that identify the arterial and venous endothelium prompted us to investigate their expression pattern in KS. Cell lines and primary tumor tissues expressed only the arterial endothelial marker ephrin B2. Expression of EphB4, which is the ephrin B2 cognate receptor and marker for venous endothelial cells, was notably lacking. It is known that ephrin B2 expression represses EphB4 and vice versa,19 therefore the observed findings are consistent with this reciprocal regulation and show this regulation is intact in KS cells. The arterial phenotype of KS may be broader than just ephrin B2 expression since KS cells also express CD148, which is normally expressed in arterial endothelial cells.31
The normal capillary network of the microvasculature is composed of equal compartments of arterial capillaries expressing ephrin B2 and ephB4-expressing venous capillaries, which form regular junctions with each other.10,32 Since KS lesions consist of proliferative microvasculature, one would expect that the distribution of ephrin B2- and EphB4-expressing cells would be nearly equal. Our finding of a preponderance of ephrin B2 expression is consistent with a clonal population of neoplastic KS cells, expressing ephrin B2 and certain other arterial markers, that has evaded the normal constraints of artery-vein demarcation in the capillary bed.
HHV-8 is associated with the development of KS. Nearly all KS cells in situ express latency proteins,33 and some cells express lytic-phase proteins.34 The role of HHV-8 in the induction of ephrin B2 was tested by infection of EphB4-positive venous endothelial cells. Ephrin B2 was indeed induced and EphB4 down-regulated in the clusters of cells showing viral infection. This supports the role of HHV-8 in regulation of ephrin B2 and suggests that the presence of HHV-8 in most KS tumor cells causes the overwhelming expression of ephrin B2 and lack of EphB4.
HHV-8 could induce expression of ephrin B2 and certain other artery-specific genes via one or more of the viral proteins. Two HHV-8 genes were assessed for their effect on ephrin B2 expression. LANA1 is a latency-phase protein present in nearly all KS cells in situ, which makes it a candidate for ephrin B2 induction. LANA1, however, failed to induce ephrin B2. The effect of vGPCR expression was tested due to its ability to transform cells and induce KS-like lesions when expressed in endothelial cells of the mouse.9 We found that ectopic expression of the lytic protein vGPCR in both stable and transient systems induced ephrin B2 expression.
The obvious question is whether constitutive expression of vGPCR is required in KS tumor cells to maintain ephrin B2-positive phenotype. Widespread expression of ephrin B2 in KS tumor tissue and the fact that vGPCR expression is limited to rare cells35 argues that it is not necessary. It is possible that vGPCR induces paracrine effects via regulation of cellular genes. However, induction of ephrin B2 by HHV-8 is likely not limited to vGPCR and would include the activities of other viral proteins. KS cell lines widely used for investigation of KS lack HHV-8, consistent with the observation that KS primary cultures lose HHV-8 within a few passages. Lack of HHV-8 and continued expression of ephrin B2 suggest that the phenotype becomes fixed and independent of continued need for the presence of HHV-8 and is maintained by clonal expansion of transformed cells.
The transient expression of the lytic cycle vGPCR transcript36 makes it unlikely that vGPCR directly transforms spindle cells in vivo. However, vGPCR up-regulates the expression of genes involved in KS pathogenesis, including growth-related oncogene (GRO), IL-6, VEGF-C, and the vitamin D receptor (VDR) in the vGPCR-transfected classic KS cell line KS-SLK.37 In addition, IL-6, IL-8, RANTES (regulated on activation, normal T-expressed, and secreted), granulocyte-macrophage colony-stimulating factor (GM-CSF), and VEGF are up-regulated secondary to the regulation of nuclear factor-B (NFB) in KSIMM cells or HUVECs transfected with vGPCR.38 vGPCR is able to use multiple cellular pathways to alter endothelial cells; it enhances expression of VEGF as through p38 and MAPK stimulation of hypoxia-inducible factor 1 (HIF-1)39; and promotes endothelial cell survival in a phosphatidylinositol 3 kinase (PI3K)/AKT-dependent manner.40 In addition, HHV-8 induces VEGFR-2 thus conferring increased sensitivity to VEGF.7,41 In the context of ephrin B2 expression, it is notable that vGPCR induces expression of VEGF and VEGFR-2, since VEGF is necessary for ephrin B2 expression and maintenance of the arterial phenotype.30,42 Consistent with this we show that VEGF is required for up-regulation of ephrin B2 in KS cells expressing vGPCR. However, we also document the contribution of other KS growth factors, notably VEGF-C, IL-8, and IL-6, to ephrin B2 expression. Neutralization of these endogenously expressed factors by antibodies or soluble receptors all decreased ephrin B2 expression and viability in KS-SLK cells expressing vGPCR. Conversely, VEGF, VEGF-C, IL-8, and IL-6 were all able to induce ephrin B2 expression in venous endothelial cells. These data support the indirect/paracrine effects of vGPCR in inducing ephrin B2 and provide the first demonstration of the involvement of VEGF-C, IL-8, and IL-6 in arterial marker gene expression. KS cell lines express high levels of VEGF, VEGF-C, IL-8, IL-6, and their cognate receptors. It is thus likely that the ephrin B2 phenotype is maintained via constitutive activation of these receptors. The role of other KS growth factors, such as tumor necrosis factor (TNF-), bFGF, and IL-1, in altering ephrin B2 and EphB4 expression is under investigation.
In the context of HIV infection, the activation of VEGF receptors by HIV Tat protein43 would complement this phenotype. In addition, HIV induces several cytokines that are known to enhance VEGF expression, including IL-1 and TNF- in selected cell types.44 HHV-8 and HIV are thus likely to induce the observed phenotype in AIDS-KS via viral and cellular proteins. Whether HIV Tat can itself induce ephrin B2 expression in our system and whether it potentiates the demonstrated effect of HHV-8 vGPCR is the subject of ongoing investigations.
We showed that ephrin B2 expression is necessary for survival of KS cells. Knock down of ephrin B2 expression by specific siRNA reduced KS cell viability. Further, ephrin B2 knockdown resulted in loss of endothelial cell viability when cultured in VEGF (conditions that induce the expression of ephrin B2) but not of the same cells cultured in the absence of VEGF and presence of IGF, EGF, and bFGF. This indicates that once the arterial phenotype is expressed, the continued presence of ephrin B2 is required for cell viability. This is the first demonstration of a direct requirement for the expression of ephrin B2 for cell viability. Ablation of ephrin B2 expression activated caspase 8 and led to apoptosis. Caspase 8 activation is indicative of cell death receptor-mediated apoptosis, which is initiated by the formation of a death-inducing signaling complex (DISC) at the cell membrane (reviewed in Curtin and Cotter45). Examples of death receptors are Fas/CD95/Apo1, TNF-R1, and TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1). Notably, both Fas and ephrin B2 have been shown to bind the protein tyrosine phosphatase (PTP)-basophil/basophil-like (PTB-BL), which is a central cytoskeletal scaffolding protein (reviewed in Erdman46). This raises the intriguing possibility that high-level expression of ephrin B2 in KS inhibits the formation of Fas DISC hexamers through interaction with PTB-BL, thus tipping the balance away from apoptosis. By ablation of ephrin B2 in this setting, we may have swung the balance in favor of DISC formation and allowed apoptosis. This possibility is under formal investigation.
It is also known that reverse signaling from ephrin B2 stimulates migration and sprouting angiogenesis.21,22 This suggests that ephrin B2 expression in KS has functional consequences for KS pathology and further that ephrin B2 is a valid therapeutic target in this disease.
Footnotes
Prepublished online as Blood First Edition Paper, October 7, 2004; DOI 10.1182/blood-2004-03-0933.
Supported by research grant from National Cancer Institute 1R01 CA 79218 (P.S.G.).
An Inside Blood analysis of this article appears in the front of this issue.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
References
Gill PS, Hamilton A, Naidu Y. Epidemic (AIDS-related) Kaposi's sarcoma: epidemiology pathogenesis and treatment. AIDS Updates. 1994;7: 1-11.
Lunardi-Iskandar Y, Gill PS, Lam VH, et al. Isolation and characterization of an immortal neoplastic cell line (KS Y-1) from AIDS-associated Kaposi's sarcoma. J Natl Can Inst. 1995;87: 974-981.
Masood R, Cai J, Zheng T, Smith DL, Naidu Y, Gill PS. Vascular endothelial growth factor/vascular permeability factor is an autocrine growth factor for AIDS-Kaposi sarcoma. Proc Natl Acad Sci U S A. 1997;94: 979-984.
Weninger W, Partanen TA, Breiteneder-Geleff S, et al. Expression of vascular endothelial growth factor receptor-3 and podoplanin suggests a lymphatic endothelial cell origin of Kaposi's sarcoma tumor cells. Lab Invest. 1999;79: 243-251.
Chang Y, Cesarman E, Pessin ME, et al. Identification of herpes virus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science. 1994;266: 1865-1869.
Ganem D, Kedes DH, Operskalski E, et al. The seroepidemiology of human herpes virus 8 (Kaposi's sarcoma associated herpes virus): distribution of infection in KS risk groups and evidence for sexual transmission. Nat Med. 1996;2: 918-924.
Flore O, Rafii S, Ely S, O'Leary JJ, Hyjek EM, Ceasarman E. Transformation of primary human endothelial cells by Kaposi's sarcoma-associated herpesvirus. Nature. 1998;394: 588-592.
Mihalcea AM, Smith DL, Monini P, Sgadari C, Ensoli B, Gill PS. Treatment update for AIDS-related Kaposi's sarcoma. AIDS. 1999;13: S215-S225.
Montaner S, Sodhi A, Molinolo A, et al. Endothelial infection with KSHV genes in vivo reveals that vGPCR initiates Kaposi's sarcomagenesis and can promote the tumorigenic potential of viral latent genes. Cancer Cell. 2003;3: 23-36.
Adams RH, Wilkinson GA, Weiss C, et al. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 1999;13: 295-306.
Gerety SS, Wang HU, Chen ZF, Anderson DJ. Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development. Mol Cell. 1999;4: 403-414.
Wang HU, Chen ZF, Anderson DJ. Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 1998;93: 741-753.
Davis S, Gale NW, Aldrich TH, et al. Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science. 1994;266: 816-819.
Kalo MS, Pasquale EB. Multiple in vivo tyrosine phosphorylation sites in EphB receptors. Biochemistry. 1999;38: 14396-14408.
Binns KL, Taylor PP, Sicheri F, Pawson T, Holland SJ. Phosphorylation of tyrosine residues in the kinase domain and juxtamembrane region regulates the biological and catalytic activities of Eph receptors. Mol Cell Biol. 2000;20: 4791-4805.
Bruckner K, Pasquale EB, Klein R. Tyrosine phosphorylation of transmembrane ligands for Eph receptors. Science. 1997;275: 1640-1643.
Palmer A, Zimmer M, Erdmann KS, et al. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Mol Cell. 2002;9: 725-737.
Sheng M, Sala C. PDZ domains and the organization of supramolecular complexes. Annu Rev Neurosci. 2001;24: 1-29.
Zhong TP, Childs S, Leu JP, Fishman MC. Gridlock signalling pathway fashions the first embryonic artery. Nature. 2001;414: 216-220.
Shin D, Garcia-Cardena G, Hayashi S, et al. Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 2001;230: 139-150.
Hamada K, Oike Y, Ito Y, et al. Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells. Arterioscler Thromb Vasc Biol. 2003;23: 190-197.
Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J Cell Sci. 2003;116: 2461-2470.
Liu ZJ, Shirakawa T, Li Y, et al. Regulation of Notch1 and D114 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol Cell Biol. 2003;23: 14-25.
D'Amore PA, Ng Y-S. Won't you be my neighbor Local induction of arteriogenesis. Cell. 2002;110: 289-292.
Arvanitakis L, Mesri EA, Nador RG, et al. Establishment and characterization of a primary effusion (body cavity-based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood. 1996;88: 2648-2654.
Siegal B, Levinton-Kriss S, Schiffer A, et al. Kaposi's sarcoma in immunosuppression: possibly the result of a dual viral infection. Cancer. 1990;65: 492-498.
Xia G, Kageyama Y, Hayashi T, Kawakami S, Yoshida M, Kihara K. Regulation of vascular endothelial growth factor transcription by endothelial PAS domain protein 1 (EPAS1) and possible involvement of EPAS1 in the angiogenesis of renal cell carcinoma. Cancer. 2001;91: 1429-1436.
Sakurada S, Katano H, Sata T, Ohkuni H, Watanabe T, Mori S. Effective human herpesvirus 8 infection of human umbilical vein endothelial cells by cell-mediated transmission. J Virol. 2001;75: 7717-7722.
Masood R, Cai J, Zheng T, Smith DL, Hinton DR, Gill PS. Vascular endothelial growth factor (VEGF) is an autocrine growth factor for VEGF receptor-positive human tumors. Blood. 2001;98: 1904-1913.
Sinha UK, Kundra A, Scalia P, et al. Expression of EphB4 in head and neck squamous cell carcinoma. Ear Nose Throat J. 2003;82: 866, 869-70, 887.
Takahashi T, Takahashi K, Mernaugh R, et al. Endothelial localization of receptor tyrosine phosphatase, ECRTP/DEP-1, in developing and mature renal vasculature. J Am Soc Nephrol. 1999; 10: 2135-2145.
Mukouyama Y, Shin D, Britsch S, Taniguchi M, Anderson DJ. Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell. 2002;109: 693-705.
Dupin N, Fisher C, Kellam P, et al. Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma. Proc Natl Acad Sci U S A. 1999;196: 4546-4551.
Ascherl G, Hohenadl C, Monini P, et al. Expression of human herpesvirus-8 (HHV-8) encoded pathogenic genes in Kaposi's sarcoma (KS) primary lesions. Adv Enzyme Regul. 1999;39: 331-339.
Kirshner JR, Staskus K, Haase A, Lagunoff M, Ganem D. Expression of the open reading frame 74 (G-protein-coupled receptor) gene of Kaposi's sarcoma (KS)-associated herpesvirus: implications for KS pathogenesis. J Virol. 1999;73: 6006-6014.
Dezube BJ, Zambela M, Sage DR, Wang JF, Fingeroth JD. Characterization of Kaposi sarcoma-associated herpesvirus/human herpesvirus-8 infection of human vascular endothelial cells: early events. Blood. 2002;100: 888-896.
Polson AG, Wang D, DeRisi J, Ganem D. Modulation of host gene expression by the constitutively active G protein-coupled receptor of Kaposi's sarcoma-associated herpesvirus. Cancer Res. 2002;62: 4525-4530.
Pati S, Cavrois M, Guo HG, et al. Activation of NF-kappaB by the human herpesvirus 8 chemokine receptor ORF74: evidence for a paracrine model of Kaposi's sarcoma pathogenesis. J Virol. 2001;75: 8660-8673.
Sodhi A, Montaner S, Patel V, et al. The Kaposi's sarcoma-associated herpes virus G protein-coupled receptor up-regulates vascular endothelial growth factor expression and secretion through mitogen-activated protein kinase and p38 pathways acting on hypoxia-inducible factor 1 alpha. Cancer Res. 2000;60: 4873-4880.
Montaner S, Sodhi A, Pece S, Mesri EA, Gutkind JS. The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor promotes endothelial cell survival through the activation of Akt/protein kinase B. Cancer Res. 2001;61: 2641-2648.
Moses AV, Jarvis MA, Raggo C, et al. Kaposi's sarcoma-associated herpesvirus-induced up-regulation of the c-kit proto-oncogene, as identified by gene expression profiling, is essential for the transformation of endothelial cells. J Virol. 2002;76: 8383-8399.
Lawson ND, Vogel AM, Weinstein BM. Sonic hedgehog and vascular endothelial growth factor act upstream of the notch pathway during arterial endothelial differentiation. Dev Cell. 2002;3: 127-136.
Albini A, Soldi R, Giunciuglio D, et al. The angiogenesis induced by HIV-1 tat protein is mediated by the Flk-1/KDR receptor on vascular endothelial cells. Nat Med. 1996;2: 1371-1375.
Barillari G, Sgadari C, Palladino C, et al. Inflammatory cytokines synergize with the HIV-1 Tat protein to promote angiogenesis and Kaposi's sarcoma via induction of basic fibroblast growth factor and the alpha v beta 3 integrin. J Immunol. 1999;163: 1929-1935.
Curtin JF, Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003;15: 983-992.
Erdman KS. The protein tyrosine phosphatase PTP-basophil/basophil-like: interacting proteins and molecular functions. Eur J Biochem. 2003; 270: 4789-4798.(Rizwan Masood, Guangbin X)
Abstract
Kaposi sarcoma (KS) is an angioproliferative tumor derived from endothelial cells in which tumor cells form aberrant vascular structures. Ephrin B2 and ephrin B4 (EphB4) are artery- and vein-specific proteins, respectively, with critical roles in vessel maturation. We investigated whether the disorganized KS vasculature was due to unbalanced expression of ephrin B2 and EphB4. Secondly, we wished to determine if human herpesvirus type 8 (HHV-8), the viral agent associated with KS, regulates ephrin B2 and EphB4. An arterial phenotype was observed in KS tissue and cell lines, as shown by abundant expression of ephrin B2 with little or no EphB4. Infection of venous endothelial cells with HHV-8 resulted in a phenotype switch from EphB4 to ephrin B2, similar to that seen with vascular endothelial growth factor (VEGF). The HHV-8 effect on ephrin B2 expression was reproduced with the HHV-8-specific viral G-protein-coupled receptor. We also showed that ephrin B2 expression is required for KS cell viability by knock down with siRNA. KS is the first example of a human tumor with a predominantly arterial phenotype. This predominance can be attributed to expression of HHV-8 proteins and their downstream effects. Ephrin B2 is thus an important novel factor in KS biology and a potential target for therapy.
Introduction
Kaposi sarcoma (KS) manifests as a multifocal angioproliferative disease, most commonly of the skin and mucus membranes with subsequent spread to visceral organs.1 Hallmarks of the disease are angiogenesis, edema, infiltration of lymphomononuclear cells, and growth of spindle-shaped tumor cells. Pathologically, established lesions exhibit an extensive vascular network of slitlike spaces. Abnormal spindle-shaped endothelial cells (tumor cells) line the tumor vessels, which also lack basement membranes. Defective vasculature results in an accumulation of the blood components including albumin and red and mononuclear cells in the lesions.1 The KS tumor is endothelial in origin; the tumor cells express many endothelial markers, including lectin binding sites for Ulex europeaus agglutinin-1 (UEA-1), CD34, EN-4, pathologische anatomie Leiden endothelium (PAL-E),2 and the endothelial cell-specific tyrosine kinase receptors vascular endothelial growth factor receptor 1 (VEGFR-1; fetal liver tyrosine kinase 1 [Flt-1]), VEGFR-2 (fetal liver kinase-1/kinase domain receptor [Flk-1/KDR]), VEGFR-3 (Flt-4), Tie-1, and Tie-2 (Masood et al3; R.M. and P.S.G., unpublished data, 2004). KS cells coexpress lymphatic endothelial cell-related proteins including lymphatic vessel endothelial hyaluronan receptor (LYVE) and podoplanin.4
The gammaherpesvirus human herpesvirus type 8 (HHV-8) is considered the etiologic agent for the disease. In 1994 sequences of this herpes virus were identified in KS tumor tissue,5 and subsequent molecular epidemiology studies have shown that nearly all KS tumors contain viral genome. Seroepidemiology studies show that HIV-infected patients with KS have the highest prevalence of HHV-8 and secondly that those with HIV infection but no KS have increased risk of development of KS over the ensuing years if they are also seropositive for HHV-8.6 Direct evidence for the role of HHV-8 in KS is the transformation of bone marrow endothelial cells after infection with HHV-8.7 A number of HHV-8-encoded genes could contribute to cellular transformation (reviewed in Mihalcea et al8). However, the most evidence has accumulated for the viral G-protein-coupled receptor (vGPCR) in this role.9
Vascular endothelium is functionally and phenotypically divided into 2 distinct populations. Venous endothelial cells express the tyrosine kinase receptor EphB4, whereas arterial endothelial cells express its membrane-bound ligand ephrin B2.10-12 Cell-cell contact is required for binding and activation of Eph's by clustered membrane-attached ephrins.13 Full activation of Eph dimers is accomplished by autophosphorylation of juxtamembrane tyrosine residues.14,15 In addition to forward signaling through the Eph's, reverse signaling can occur through phosphorylation of the ephrin Bs.16-18
Targeted gene knockouts of either ephrin B2 or EphB4 in mice show similar phenotypes with disrupted vessel maturation and early embryonic lethality.11,12 Artery and vein phenotypes are determined very early in development, at the time of mesoderm differentiation to the vascular lineage.19 The ephrin B2/EphB4 ligand/receptor pair is important in vasculogenesis in the embryo and is also essential for defining the boundaries between arterial and venous domains10 that persist in the adult.20 It has been shown in vitro that EphB4-mediated forward signaling restricts intermingling of cells and supports cellular segregation, whereas reverse signaling from ephrin B2 stimulates migration and sprouting angiogenesis.21,22
The current model for artery/vein differentiation is that venous differentiation is the default pathway. VEGF appears to be a permissive signal for attaining the arterial phenotype through up-regulation of the Notch receptor and its ligand delta-like 4 (Dll-4) in arterial endothelial cells.23,24
We investigated whether KS tumor cells are derived from arterial or venous endothelium. In addition, we investigated whether HHV-8 has an effect on expression of arterial or venous markers in a model of KS. KS tumor cells were found to express the ephrin B2 arterial marker. Further, ephrin B2 expression was induced by HHV-8 and also by the HHV-8-specific vGPCR in KS and endothelial cell lines. Ephrin B2 is a potential target for treatment of KS because inhibition of ephrin B2 expression or signaling was detrimental to KS cell viability and function.
Patients, materials, and methods
Cell lines and reagents
Human umbilical vascular endothelial cells (HUVECs) were from Clonetics (San Diego, CA) and were maintained in endothelial growth medium 2 (EGM-2; Clonetics). EGM-2 is supplied as endothelial basal medium 2 (EBM-2) with supplements for fresh addition: fetal calf serum (FCS) to 2%, human epidermal growth factor (hEGF), hydrocortisone, VEGF, basic fibroblast growth factor (bFGF), recombinant insulin-like growth factor 1 (IGF-1), ascorbic acid, heparin, gentamicin, and Amphotericin-B. T1 human fibroblast line was from Dr Peter Jones (University of Southern California [USC]). BC-1 (HHV-8-positive, Epstein-Barr virus [EBV]-positive) and BC-3 (HHV-8-positive, EBV-negative) human pleural effusion lymphoma cell lines25 and monoclonal antibodies to latency-associated nuclear antigen 1 (LANA1) and HHV-8 lytic-phase ORF59 protein (ORF59) were the kind gift of Dr Dharam Ablashi (Advanced Biotechnologies, Columbia, MD). The KS-SLK cell line was isolated from a patient with classic KS.26 SCC-25 and SCC-15 cell lines were from the American Type Culture Collection (ATCC; Manassas VA). Antibodies to EphB4, ephrin B2, CD148, platelet endothelial cell adhesion molecule 1 (PECAM-1; all polyclonal), and VEGFR-2 (monoclonal) were from Santa Cruz Biotechnology (Santa Cruz, CA). VEGFR-3/Fc and monoclonal antibodies to human VEGF, VEGF-C, interleukin-6 (IL-6), IL-8, and oncostatin-M were from R&D Systems (Minneapolis, MN). Antibodies to p44/42 mitogen-activated protein kinase (MAPK; polyclonal) and phospho-p44/42 MAPK (monoclonal) were from New England Biolabs (Beverly, MA), and antiphosphotyrosine monoclonal antibody was from Upstate (Charlotte, VA). Expression vectors pCEFL-KS-associated herpesvirus-GPCR (pCEFL-KSHV-GPCR) and pCEFL were the kind gift of Dr Enrique Mesri (Cornell University, NewYork, NY). Expression vectors for HHV-8 LANAand LANA440, which lacks the nuclear localization domain, were kindly provided by Dr Matthew Rettig, Veteran's Administration Greater LosAngeles Healthcare System.
Collection and preparation of human tissue
Human cutaneous KS biopsies were obtained under local anesthesia with informed consent from patients at the Los Angeles County (LAC)/USC Medical Center, using an institutional review board (IRB)-approved consent form. Biopsies were processed for either total RNA, paraffin blocks, or frozen tissue blocks in optimum cutting temperature (OCT). Total RNA was extracted by homogenization in RNAzol (Tel-Test, Friendswood, TX). cDNAs were synthesized by reverse transcriptase using a random hexamer primer (Superscript II; Invitrogen, Carlsbad, CA).
Preparation of digoxigenin-labeled RNA probes and in situ hybridization
Ephrin B2 and EphB4 polymerase chain reaction (PCR) products from the primers shown in Table 1 for in situ hybridization (ISH) were cloned into pGEM-T Easy Vector System (Promega, Madison, WI). DNA sequencing confirmed the orientation and authenticity of the inserts. Antisense or sense digoxigenin (DIG)-labeled RNA probes were created by run-off transcription from T7 or SP6 promoters using a DIG RNA labeling kit (Roche, Indianapolis, IN). RNA probes were quantitated by spot assay according to the manufacturer's instructions.
Frozen sections were subject to ISH essentially as previously described,27 except that the hybridization step was overnight at 42°C with 25 ng antisense or sense RNAprobes. Hybridization signal was detected using alkaline-phosphatase-conjugated anti-DIG antibodies (Roche) according to the manufacturer's instructions. Cells were visualized by counterstaining of nucleic acids with Nuclear Fast Red (Vector Laboratories, Burlingame, CA).
Coculture of HUVECs and BC-1
HUVECs were grown to 50% to 70% confluence in EGM-2 on gelatin-coated chamber slides (Nalge Nunc International, Naperville, IL). Coculture with BC-1 or BC-3 was essentially as described by Sakurada et al.28 Briefly, BC-1 or BC-3 cells were pretreated with TPA (12-O-tetradecanoyl-phorbol 13-acetate; 20 ng/mL) to induce virus for 48 hours and then added to the HUVEC culture at a ratio of 10:1 for cocultivation for 2 days. The HUVECs were washed extensively with phosphate-buffered saline (PBS) to remove the attached BC-1 or BC-3 cells.
Preparation of cDNA and RT-PCR
Cell viability assay
KS-SLK cells were seeded at a density of 1 x 104 per well in 48-well plates on day 0 in appropriate growth medium containing 2% FCS. On the following day, the medium was changed and cells were transfected with 0, 10, or 100 nM siRNA. Forty-eight hours after transfection, viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) as previously described.29 A similar procedure was followed for studies with neutralizing antibody and VEGFR-3/Fc. The following day, plating medium was replaced with 100 ng/mL antibody or recombinant Fc fusion protein and viability assessed 48 hours later with MTT.
Immunofluorescence studies
Cells cultured on chamber slides or frozen sections of KS biopsy material were fixed in 4% paraformaldehyde and preincubated with blocking buffer (0.2% Triton-X100, 1% bovine serum albumin [BSA] in PBS) for 20 minutes, followed by incubation with antibodies to EphB4, ephrin B2, CD148, LANA1 or ORF59 (1:100 dilution in PBS) in blocking buffer at 4°C for 16 hours. Slides were then incubated with the appropriate fluorescein- or rhodamine-conjugated secondary antibodies (Sigma-Aldrich, St Louis, MO). Nuclei were counterstained with DAPI (4',6-diamidino-2-phenylindole dihydrochloride hydrate). Images were obtained using a Olympus AX70 fluorescence microscope and Spot v2.2.2 (Diagnostic Instruments, Sterling Heights, MI) digital imaging system.
Immunofluorescence detection of ephrin B2 with EphB4-Fc
Frozen sections fixed in 4% paraformaldehyde and blocked with 20% FCS were incubated with 5 μg/mL EphB4/Fc (R&D Systems) for 1 hour at room temperature. Sections were then incubated with 10 μg/mL rabbit anti-human immunoglobulin G-fluorescein isothiocyanate (IgG-FITC) in PBS (Jackson ImmunoResearch Laboratories, West Grove, PA) at room temperature for 1 hour. Nuclei were counterstained with DAPI and sections mounted. Human IgG Fc (Jackson ImmunoResearch Laboratories) was used as the negative control.
Western blot
Crude cell lysates were prepared, quantitated, fractionated, and transferred to membranes as described previously.29 Membranes were blocked with 5% nonfat milk prior to incubation with antibody to ephrin B2 (1:5000 dilution) at 4°C for 16 hours. Secondary antibody (1:5000 dilution) conjugated with horseradish peroxidase was applied for 1 hour at 25°C. The membranes were developed using the SuperSignal West Femto Maximum sensitivity chemiluminescent substrate (Pierce, Rockford, IL) according to the manufacturer's instructions. Membranes were stripped using Restore Western Blot Stripping Buffer (Pierce) and reprobed with EphB4 or -actin.
Synthesis of ephrin B2 and EphB4 siRNA by in vitro transcription
Ephrin B2 or EphB4 knockdown with siRNA in HUVECs grown with or without VEGF
Duplicate cultures of HUVECs were seeded on 8-well chamber slides coated with fibronectin and grown overnight in EGM-2. Sixteen hours later, medium was replaced with basal medium (EBM-2) supplemented with 5% fetal calf serum (FCS) and EGM-2 BulletKit supplements bFGF, hEGF, and recombinant insulin-like growth factor 1 (R3-IGF-1) at the concentrations provided by the manufacturer in half of the wells or 5% FCS and 10 ng/mL recombinant human VEGF (rhVEGF; R&D Systems) in the remaining wells. After 2 hours incubation at 37°C, all cells were transfected using Lipofectamine 2000 (1 μg/mL; Invitrogen) and 10 nM specific siRNAs in Opti-MEM-1 serum-free medium (Invitrogen). Following transfection for 2 hours in Opti-MEM-1, EBM-2 medium containing growth supplements was replaced in the appropriate wells. After 48 hours, the cells were stained with crystal violet and immediately photographed at x 10 magnification.
Construction of ephrin B2 reporter plasmids
Human ephrin B2 5'-flanking DNA from -2491 to -11 with respect to the translation start site was amplified from BACPAC clone RP11-297I6 (BacPac Resources, Children's Hospital, Oakland, CA) using theAdvantage GC Genomic PCR kit (Clontech) to overcome the large tracts of GC-rich sequence in the target area. Primers were designed to contain MluI sites for cloning into pGL3Basic Vector (Promega). Orientation of the resulting clones was confirmed by restriction digest analysis. The correct clone was designated pEFNB2-2491/-11luc. Digestion of this clone with either KpnIor SacI followed by recircularization yielded pEFNB2-1242/-11luc and pEFNB2-577/-11luc, respectively. Plasmid DNAs used for transient transfections were purified using a Mega Prep kit (QIAGEN, Valencia, CA).
Transient transfection and reporter assays
HUVECs (0.8 x 104 cells/well in 24-well plates) maintained in EGM-2 media were transiently cotransfected with 0.5 μg/well ephrin B2 promoter-luciferase constructs together with 50 ng/well either pCEFL or pCEFL-KSHV-GPCR, using Superfect reagent (QIAGEN) according to the manufacturer's instructions. Cells were harvested 48 hours after transfection and lysed with Luciferase cell lysis buffer (Promega). Luciferase activity was assayed using the Luciferase Assay System (Promega) according to the manufacturer's instructions. Luciferase was normalized to protein because pCEFL-vGPCR induced the expression of -galactosidase from pCMV-Sport-gal (Invitrogen).
Apoptosis enzyme-linked immunosorbent assay (ELISA) and caspase activation assays
Apoptosis was detected using the Cell Death Detection ELISAPlus Kit (Roche) following the manufacturer's instructions 48 hours after siRNA transfection of 2500 KS-SLK cells/well. For caspase activation assays, 2.5 x 106 cells were transfected with siRNAs. Forty-eight hours after transfection, activation of caspases 8 and 9 were determined on cytosolic extracts using the caspase 8 and caspase 9 colorimetric assays (R&D Systems) according to the manufacturer's instructions.
Results
Ephrin B2 but not EphB4 transcripts in KS tumors
The highly vascular nature of KS lesions and the probable endothelial cell origin of the tumor cells prompted investigation of expression of EphB4 and ephrin B2, which are markers for venous and arterial endothelial cells, respectively. Ephrin B2 but not EphB4 transcripts were detected in tumor cells of KS biopsies by in situ hybridization (Figure 1A). Comparison of the positive signal with ephrin B2 antisense probe and tumor cells as shown by hematoxylin and eosin (H&E) staining shows that ephrin B2 expression is limited to the areas of the biopsy that contain tumor cells. Specificity of the EphB4 antisense probe was confirmed by positive signal for transcripts in head and neck squamous cell carcinoma (HNSCC), which we have shown expresses EphB4.30
EphB4 binding sites in KS tumors
Additional evidence for the expression of ephrin B2 in KS tumor tissue is afforded by the localization of EphB4/Fc signal to tumor cells detected by FITC-conjugated anti-human Fc antibody. Because ephrin B2 is the only ligand for EphB4, this reagent is specific for the expression of ephrin B2 (Figure 1Bi). An adjacent section treated only with human Fc and the secondary reagents shows no specific signal.
Coexpression of ephrin B2 and HHV-8 proteins in KS tumors
In other studies, 2-color confocal microscopy demonstrated the presence of the HHV-8 latency protein LANA1 in the ephrin B2-positive cells (Figure 1Ci), indicating that it is the tumor cells, not tumor vessels, that are expressing this arterial marker. Staining of tumor biopsy with the vessel-specific PECAM-1/CD31 antibody revealed the highly vascular nature of this tumor (Figure 1Ciii).
Ephrin B2 but not EphB4 is present in KS biopsies and cell lines
Western blots on KS biopsy lysates confirmed the expression of ephrin B2 but not EphB4. Matched normal skin was obtained for 2 of the cases and these expressed neither ephrin B2 nor EphB4 (Figure 1D). In addition, the immortalized KS cell line KS-SLK and a primary isolate, KS 6-3, showed the same pattern of expression as the biopsy material. The EphB4 antibody could detect protein, as shown by signal in squamous cell carcinomas SCC-15 and SCC-25.
Arterial phenotype switch in venous endothelial cells infected with HHV-8
We next asked whether HHV-8, the presumed etiologic agent for KS, itself induced expression of ephrin B2 and repressed EphB4 expression in endothelial cells. Coculture of HUVECs and BC-1 lymphoma cells, which are productively infected with HHV-8, results in effective infection of the endothelial cells.28 The attached monolayers of endothelial cells remaining after extensive washing were examined for ephrin B2 and EphB4 by RT-PCR and immunofluorescence. HUVECs express EphB4 venous marker strongly at the RNA level but not ephrin B2 (Figure 2A). In contrast, HHV-8-infected cultures (HUVEC/BC-1 and HUVEC/BC-3) express ephrin B2, whereas EphB4 transcripts are almost absent. Ephrin B2 signal in infected cell cultures is not due to residual BC-1 cells, as they do not express ephrin B2 and only express low levels of EphB4 mRNA (Figure 2A). No evidence of ephrin B2 and EphB4 protein in BC-1 and BC-3 cell lines was found by Western blot (Figure 2B). Positive controls for the antibodies run on the same gel are shown in Figure 1D (SCC-15).
Coexpression of arterial markers and HHV-8 proteins in infected endothelial cells
Immunofluorescence analysis of cultures of HUVECs and HUVEC/HHV-8 for artery/vein markers and viral proteins was undertaken to determine whether changes in protein expression mirrored that seen in the RNA. In addition, cellular localization of the proteins could be determined. Consistent with the RT-PCR data, HUVECs are ephrin B2 negative and EphB4 positive (Figure 2Ci,xiii). As expected, they do not express any HHV-8 LANA1 (Figure 2Cii,xiv). Coculture of BC-1 cells, which are productively infected with HHV-8, resulted in infection of HUVECs as shown by presence of viral proteins LANA1 and ORF59 (Figure 2Cvi,xviii). HHV-8-infected HUVECs now express ephrin B2 but not EphB4 (Figure 2Cv,xvii,xxi, respectively). Expression of ephrin B2 and LANA1 cocluster is shown by yellow signal in the merged image (Figure 2Cviii). HHV-8-infected HUVECs positive for ephrin B2 and negative for EphB4 also express the arterial marker CD14819 (Figure 2Cx,xxii). Expression of ephrin B2 and CD148 cocluster is shown by yellow signal in the merged image (Figure 2Cxii). Uninfected HUVECs expressing EphB4 were negative for CD148 (not shown).
HHV-8 vGPCR induces ephrin B2 protein
To test whether individual viral proteins could induce the expression of ephrin B2 seen with the whole virus, KS-SLK cells were stably transfected with HHV-8 LANA, LANA440 (which lacks the nuclear localization domain), or vGPCR. Western blot of stable clones showed overexpression of ephrin B2 in KS-SLK cells transfected with vGPCR compared with LANA, LANA440, or vector alone (pCEFL; Figure 3A). Expression of HHV-8 proteins in stable clones was confirmed by RT-PCR (Figure 3B). Transient transfection of KS-SLK cells with vGPCR resulted in induction of ephrin B2 expression as detected by immunofluorescence (Figure 3C) compared with vector alone (pCEFL). EphB4 was not induced in KS-SLK-vGPCR compared with KS-SLK-pCEFL, and negligible EphB4 expression in KS-SLK cells (data not shown) precluded determination of whether it was repressed in the KS-SLK-vGPCR transfectants.
Transcriptional regulation of ephrin B2 by HHV-8 vGPCR
Since we had shown that HHV-8 induced expression of ephrin B2 in HUVECs, we next asked if this could be mediated by a transcriptional effect. Ephrin B2 5'-flanking DNA-luciferase reporter plasmids were constructed as described in "Patients, materials, and methods" and transiently transfected into HUVECs. Ephrin B2 5'-flanking DNA sequences -2491/-11 have minimal activity in HUVECs, consistent with ephrin B2 being an arterial not venous marker. However, we have noted that HUVECs in culture do express some ephrin B2 RNA. Cotransfection of HHV-8 vGPCR induces ephrin B2 transcription approximately 10-fold above background compared with the control expression vector pCEFL (Figure 3D). Roughly equal induction was seen with ephrin B2 sequences -2491/-11, -1242/-11, or -577/-11, which indicates that elements between -577 and -11 are sufficient to mediate the response to vGPCR.
Expression of ephrin B2 is regulated by KS growth factors
We next asked whether known KS growth factors could be involved in the vGPCR-mediated induction of ephrin B2 expression. KS-SLK-vGPCR stable transfectants were treated with neutralizing antibodies to oncostatin-M, IL-6, IL-8, VEGF, or VEGF-C for 36 hours. Neutralization of VEGF almost completely blocked expression of ephrin B2 in KS-SLK-vGPCR cells (Figure 4A). A lesser but significant decrease in ephrin B2 was seen with the IL-8, IL-6, and VEGF-C antibody treatments. No appreciable effect was seen with neutralization of oncostatin-M. The biochemical activity of the VEGF neutralizing antibody in these cells was verified by inhibition of VEGFR-2 and p44/42 MAPK phosphorylation (Figure 4B). The biologic activity of antibody treatment was illustrated by decreased cell viability in SLK cells but not in unrelated T1 fibroblasts or squamous cell carcinoma SCC-25 (Figure 4C). A soluble form of the VEGF-C receptor, VEGFR-3/Fc, was used to interfere with VEGF-C stimulation of the cells in lieu of the antibody, which is not neutralizing. VEGF, VEGF-C, IL-8, and IL-6 induced expression of ephrin B2 in HUVECs, whereas EGF and oncostatin-M did not (Figure 4D), thus providing additional evidence that these KS growth factors are integral to the induction of ephrin B2.
Ephrin B2 is necessary for KS and EC viability
Three ephrin B2 siRNAs were synthesized and transfected into KS-SLK cells. Forty-eight-hour treatment with ephrin B2 siRNAs 137 or 254 knocked down ephrin B2 protein by about 70% and inhibited KS-SLK cell viability, whereas ephrin B2 siRNA 63, EphB4 siRNA 50, or scrambled GFP siRNA had little or no effect (Figure 5A-B). Additionally, apoptosis was increased with respect to both mock-treated (Lipofectamine 2000 alone) and control siRNAs (GFPsi and EphB4 si50) with the 2 active ephrin B2 siRNAs (si137 and si254) in KS-SLK and 2 early passage KS cell isolates to a similar degree (Figure 5C). The mechanism of apoptosis was determined to be via the extrinsic or death-receptor-mediated pathway as shown by increase in activation of caspase 8. Under the same conditions, caspase 9 (mitochondrial or intrinsic pathway) was not activated (Figure 5D). KS-SLK cells express high levels of ephrin B2 and these results show that maintenance of ephrin B2 expression is integral to cell viability in this setting.
HUVECs do not express ephrin B2 (Figure 2A), except when stimulated by VEGF as shown in Figure 4B. Ephrin B2 siRNA 254 knocked down expression of ephrin B2 in VEGF-stimulated HUVECs (Figure 5E) and dramatically reduced the number of HUVECs cultured with VEGF as the sole growth factor (Figure 5F). In contrast, no appreciable effect was seen when HUVECs were cultured with a combination of IGF, EGF, and bFGF. As controls, EphB4 siRNA 50 and GFP siRNA had no detrimental effect on HUVECs in either culture condition.
Discussion
KS is a vascular proliferative disorder with tumor cells derived from endothelial cells. Recent identification of phenotypic markers that identify the arterial and venous endothelium prompted us to investigate their expression pattern in KS. Cell lines and primary tumor tissues expressed only the arterial endothelial marker ephrin B2. Expression of EphB4, which is the ephrin B2 cognate receptor and marker for venous endothelial cells, was notably lacking. It is known that ephrin B2 expression represses EphB4 and vice versa,19 therefore the observed findings are consistent with this reciprocal regulation and show this regulation is intact in KS cells. The arterial phenotype of KS may be broader than just ephrin B2 expression since KS cells also express CD148, which is normally expressed in arterial endothelial cells.31
The normal capillary network of the microvasculature is composed of equal compartments of arterial capillaries expressing ephrin B2 and ephB4-expressing venous capillaries, which form regular junctions with each other.10,32 Since KS lesions consist of proliferative microvasculature, one would expect that the distribution of ephrin B2- and EphB4-expressing cells would be nearly equal. Our finding of a preponderance of ephrin B2 expression is consistent with a clonal population of neoplastic KS cells, expressing ephrin B2 and certain other arterial markers, that has evaded the normal constraints of artery-vein demarcation in the capillary bed.
HHV-8 is associated with the development of KS. Nearly all KS cells in situ express latency proteins,33 and some cells express lytic-phase proteins.34 The role of HHV-8 in the induction of ephrin B2 was tested by infection of EphB4-positive venous endothelial cells. Ephrin B2 was indeed induced and EphB4 down-regulated in the clusters of cells showing viral infection. This supports the role of HHV-8 in regulation of ephrin B2 and suggests that the presence of HHV-8 in most KS tumor cells causes the overwhelming expression of ephrin B2 and lack of EphB4.
HHV-8 could induce expression of ephrin B2 and certain other artery-specific genes via one or more of the viral proteins. Two HHV-8 genes were assessed for their effect on ephrin B2 expression. LANA1 is a latency-phase protein present in nearly all KS cells in situ, which makes it a candidate for ephrin B2 induction. LANA1, however, failed to induce ephrin B2. The effect of vGPCR expression was tested due to its ability to transform cells and induce KS-like lesions when expressed in endothelial cells of the mouse.9 We found that ectopic expression of the lytic protein vGPCR in both stable and transient systems induced ephrin B2 expression.
The obvious question is whether constitutive expression of vGPCR is required in KS tumor cells to maintain ephrin B2-positive phenotype. Widespread expression of ephrin B2 in KS tumor tissue and the fact that vGPCR expression is limited to rare cells35 argues that it is not necessary. It is possible that vGPCR induces paracrine effects via regulation of cellular genes. However, induction of ephrin B2 by HHV-8 is likely not limited to vGPCR and would include the activities of other viral proteins. KS cell lines widely used for investigation of KS lack HHV-8, consistent with the observation that KS primary cultures lose HHV-8 within a few passages. Lack of HHV-8 and continued expression of ephrin B2 suggest that the phenotype becomes fixed and independent of continued need for the presence of HHV-8 and is maintained by clonal expansion of transformed cells.
The transient expression of the lytic cycle vGPCR transcript36 makes it unlikely that vGPCR directly transforms spindle cells in vivo. However, vGPCR up-regulates the expression of genes involved in KS pathogenesis, including growth-related oncogene (GRO), IL-6, VEGF-C, and the vitamin D receptor (VDR) in the vGPCR-transfected classic KS cell line KS-SLK.37 In addition, IL-6, IL-8, RANTES (regulated on activation, normal T-expressed, and secreted), granulocyte-macrophage colony-stimulating factor (GM-CSF), and VEGF are up-regulated secondary to the regulation of nuclear factor-B (NFB) in KSIMM cells or HUVECs transfected with vGPCR.38 vGPCR is able to use multiple cellular pathways to alter endothelial cells; it enhances expression of VEGF as through p38 and MAPK stimulation of hypoxia-inducible factor 1 (HIF-1)39; and promotes endothelial cell survival in a phosphatidylinositol 3 kinase (PI3K)/AKT-dependent manner.40 In addition, HHV-8 induces VEGFR-2 thus conferring increased sensitivity to VEGF.7,41 In the context of ephrin B2 expression, it is notable that vGPCR induces expression of VEGF and VEGFR-2, since VEGF is necessary for ephrin B2 expression and maintenance of the arterial phenotype.30,42 Consistent with this we show that VEGF is required for up-regulation of ephrin B2 in KS cells expressing vGPCR. However, we also document the contribution of other KS growth factors, notably VEGF-C, IL-8, and IL-6, to ephrin B2 expression. Neutralization of these endogenously expressed factors by antibodies or soluble receptors all decreased ephrin B2 expression and viability in KS-SLK cells expressing vGPCR. Conversely, VEGF, VEGF-C, IL-8, and IL-6 were all able to induce ephrin B2 expression in venous endothelial cells. These data support the indirect/paracrine effects of vGPCR in inducing ephrin B2 and provide the first demonstration of the involvement of VEGF-C, IL-8, and IL-6 in arterial marker gene expression. KS cell lines express high levels of VEGF, VEGF-C, IL-8, IL-6, and their cognate receptors. It is thus likely that the ephrin B2 phenotype is maintained via constitutive activation of these receptors. The role of other KS growth factors, such as tumor necrosis factor (TNF-), bFGF, and IL-1, in altering ephrin B2 and EphB4 expression is under investigation.
In the context of HIV infection, the activation of VEGF receptors by HIV Tat protein43 would complement this phenotype. In addition, HIV induces several cytokines that are known to enhance VEGF expression, including IL-1 and TNF- in selected cell types.44 HHV-8 and HIV are thus likely to induce the observed phenotype in AIDS-KS via viral and cellular proteins. Whether HIV Tat can itself induce ephrin B2 expression in our system and whether it potentiates the demonstrated effect of HHV-8 vGPCR is the subject of ongoing investigations.
We showed that ephrin B2 expression is necessary for survival of KS cells. Knock down of ephrin B2 expression by specific siRNA reduced KS cell viability. Further, ephrin B2 knockdown resulted in loss of endothelial cell viability when cultured in VEGF (conditions that induce the expression of ephrin B2) but not of the same cells cultured in the absence of VEGF and presence of IGF, EGF, and bFGF. This indicates that once the arterial phenotype is expressed, the continued presence of ephrin B2 is required for cell viability. This is the first demonstration of a direct requirement for the expression of ephrin B2 for cell viability. Ablation of ephrin B2 expression activated caspase 8 and led to apoptosis. Caspase 8 activation is indicative of cell death receptor-mediated apoptosis, which is initiated by the formation of a death-inducing signaling complex (DISC) at the cell membrane (reviewed in Curtin and Cotter45). Examples of death receptors are Fas/CD95/Apo1, TNF-R1, and TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1). Notably, both Fas and ephrin B2 have been shown to bind the protein tyrosine phosphatase (PTP)-basophil/basophil-like (PTB-BL), which is a central cytoskeletal scaffolding protein (reviewed in Erdman46). This raises the intriguing possibility that high-level expression of ephrin B2 in KS inhibits the formation of Fas DISC hexamers through interaction with PTB-BL, thus tipping the balance away from apoptosis. By ablation of ephrin B2 in this setting, we may have swung the balance in favor of DISC formation and allowed apoptosis. This possibility is under formal investigation.
It is also known that reverse signaling from ephrin B2 stimulates migration and sprouting angiogenesis.21,22 This suggests that ephrin B2 expression in KS has functional consequences for KS pathology and further that ephrin B2 is a valid therapeutic target in this disease.
Footnotes
Prepublished online as Blood First Edition Paper, October 7, 2004; DOI 10.1182/blood-2004-03-0933.
Supported by research grant from National Cancer Institute 1R01 CA 79218 (P.S.G.).
An Inside Blood analysis of this article appears in the front of this issue.
The publication costs of this article were defrayed in part by page charge payment. Therefore, and solely to indicate this fact, this article is hereby marked "advertisement" in accordance with 18 U.S.C. section 1734.
References
Gill PS, Hamilton A, Naidu Y. Epidemic (AIDS-related) Kaposi's sarcoma: epidemiology pathogenesis and treatment. AIDS Updates. 1994;7: 1-11.
Lunardi-Iskandar Y, Gill PS, Lam VH, et al. Isolation and characterization of an immortal neoplastic cell line (KS Y-1) from AIDS-associated Kaposi's sarcoma. J Natl Can Inst. 1995;87: 974-981.
Masood R, Cai J, Zheng T, Smith DL, Naidu Y, Gill PS. Vascular endothelial growth factor/vascular permeability factor is an autocrine growth factor for AIDS-Kaposi sarcoma. Proc Natl Acad Sci U S A. 1997;94: 979-984.
Weninger W, Partanen TA, Breiteneder-Geleff S, et al. Expression of vascular endothelial growth factor receptor-3 and podoplanin suggests a lymphatic endothelial cell origin of Kaposi's sarcoma tumor cells. Lab Invest. 1999;79: 243-251.
Chang Y, Cesarman E, Pessin ME, et al. Identification of herpes virus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science. 1994;266: 1865-1869.
Ganem D, Kedes DH, Operskalski E, et al. The seroepidemiology of human herpes virus 8 (Kaposi's sarcoma associated herpes virus): distribution of infection in KS risk groups and evidence for sexual transmission. Nat Med. 1996;2: 918-924.
Flore O, Rafii S, Ely S, O'Leary JJ, Hyjek EM, Ceasarman E. Transformation of primary human endothelial cells by Kaposi's sarcoma-associated herpesvirus. Nature. 1998;394: 588-592.
Mihalcea AM, Smith DL, Monini P, Sgadari C, Ensoli B, Gill PS. Treatment update for AIDS-related Kaposi's sarcoma. AIDS. 1999;13: S215-S225.
Montaner S, Sodhi A, Molinolo A, et al. Endothelial infection with KSHV genes in vivo reveals that vGPCR initiates Kaposi's sarcomagenesis and can promote the tumorigenic potential of viral latent genes. Cancer Cell. 2003;3: 23-36.
Adams RH, Wilkinson GA, Weiss C, et al. Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 1999;13: 295-306.
Gerety SS, Wang HU, Chen ZF, Anderson DJ. Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development. Mol Cell. 1999;4: 403-414.
Wang HU, Chen ZF, Anderson DJ. Molecular distinction and angiogenic interaction between embryonic arteries and veins revealed by ephrin-B2 and its receptor Eph-B4. Cell. 1998;93: 741-753.
Davis S, Gale NW, Aldrich TH, et al. Ligands for EPH-related receptor tyrosine kinases that require membrane attachment or clustering for activity. Science. 1994;266: 816-819.
Kalo MS, Pasquale EB. Multiple in vivo tyrosine phosphorylation sites in EphB receptors. Biochemistry. 1999;38: 14396-14408.
Binns KL, Taylor PP, Sicheri F, Pawson T, Holland SJ. Phosphorylation of tyrosine residues in the kinase domain and juxtamembrane region regulates the biological and catalytic activities of Eph receptors. Mol Cell Biol. 2000;20: 4791-4805.
Bruckner K, Pasquale EB, Klein R. Tyrosine phosphorylation of transmembrane ligands for Eph receptors. Science. 1997;275: 1640-1643.
Palmer A, Zimmer M, Erdmann KS, et al. EphrinB phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL phosphatase. Mol Cell. 2002;9: 725-737.
Sheng M, Sala C. PDZ domains and the organization of supramolecular complexes. Annu Rev Neurosci. 2001;24: 1-29.
Zhong TP, Childs S, Leu JP, Fishman MC. Gridlock signalling pathway fashions the first embryonic artery. Nature. 2001;414: 216-220.
Shin D, Garcia-Cardena G, Hayashi S, et al. Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol. 2001;230: 139-150.
Hamada K, Oike Y, Ito Y, et al. Distinct roles of ephrin-B2 forward and EphB4 reverse signaling in endothelial cells. Arterioscler Thromb Vasc Biol. 2003;23: 190-197.
Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG. Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J Cell Sci. 2003;116: 2461-2470.
Liu ZJ, Shirakawa T, Li Y, et al. Regulation of Notch1 and D114 by vascular endothelial growth factor in arterial endothelial cells: implications for modulating arteriogenesis and angiogenesis. Mol Cell Biol. 2003;23: 14-25.
D'Amore PA, Ng Y-S. Won't you be my neighbor Local induction of arteriogenesis. Cell. 2002;110: 289-292.
Arvanitakis L, Mesri EA, Nador RG, et al. Establishment and characterization of a primary effusion (body cavity-based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood. 1996;88: 2648-2654.
Siegal B, Levinton-Kriss S, Schiffer A, et al. Kaposi's sarcoma in immunosuppression: possibly the result of a dual viral infection. Cancer. 1990;65: 492-498.
Xia G, Kageyama Y, Hayashi T, Kawakami S, Yoshida M, Kihara K. Regulation of vascular endothelial growth factor transcription by endothelial PAS domain protein 1 (EPAS1) and possible involvement of EPAS1 in the angiogenesis of renal cell carcinoma. Cancer. 2001;91: 1429-1436.
Sakurada S, Katano H, Sata T, Ohkuni H, Watanabe T, Mori S. Effective human herpesvirus 8 infection of human umbilical vein endothelial cells by cell-mediated transmission. J Virol. 2001;75: 7717-7722.
Masood R, Cai J, Zheng T, Smith DL, Hinton DR, Gill PS. Vascular endothelial growth factor (VEGF) is an autocrine growth factor for VEGF receptor-positive human tumors. Blood. 2001;98: 1904-1913.
Sinha UK, Kundra A, Scalia P, et al. Expression of EphB4 in head and neck squamous cell carcinoma. Ear Nose Throat J. 2003;82: 866, 869-70, 887.
Takahashi T, Takahashi K, Mernaugh R, et al. Endothelial localization of receptor tyrosine phosphatase, ECRTP/DEP-1, in developing and mature renal vasculature. J Am Soc Nephrol. 1999; 10: 2135-2145.
Mukouyama Y, Shin D, Britsch S, Taniguchi M, Anderson DJ. Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell. 2002;109: 693-705.
Dupin N, Fisher C, Kellam P, et al. Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma. Proc Natl Acad Sci U S A. 1999;196: 4546-4551.
Ascherl G, Hohenadl C, Monini P, et al. Expression of human herpesvirus-8 (HHV-8) encoded pathogenic genes in Kaposi's sarcoma (KS) primary lesions. Adv Enzyme Regul. 1999;39: 331-339.
Kirshner JR, Staskus K, Haase A, Lagunoff M, Ganem D. Expression of the open reading frame 74 (G-protein-coupled receptor) gene of Kaposi's sarcoma (KS)-associated herpesvirus: implications for KS pathogenesis. J Virol. 1999;73: 6006-6014.
Dezube BJ, Zambela M, Sage DR, Wang JF, Fingeroth JD. Characterization of Kaposi sarcoma-associated herpesvirus/human herpesvirus-8 infection of human vascular endothelial cells: early events. Blood. 2002;100: 888-896.
Polson AG, Wang D, DeRisi J, Ganem D. Modulation of host gene expression by the constitutively active G protein-coupled receptor of Kaposi's sarcoma-associated herpesvirus. Cancer Res. 2002;62: 4525-4530.
Pati S, Cavrois M, Guo HG, et al. Activation of NF-kappaB by the human herpesvirus 8 chemokine receptor ORF74: evidence for a paracrine model of Kaposi's sarcoma pathogenesis. J Virol. 2001;75: 8660-8673.
Sodhi A, Montaner S, Patel V, et al. The Kaposi's sarcoma-associated herpes virus G protein-coupled receptor up-regulates vascular endothelial growth factor expression and secretion through mitogen-activated protein kinase and p38 pathways acting on hypoxia-inducible factor 1 alpha. Cancer Res. 2000;60: 4873-4880.
Montaner S, Sodhi A, Pece S, Mesri EA, Gutkind JS. The Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor promotes endothelial cell survival through the activation of Akt/protein kinase B. Cancer Res. 2001;61: 2641-2648.
Moses AV, Jarvis MA, Raggo C, et al. Kaposi's sarcoma-associated herpesvirus-induced up-regulation of the c-kit proto-oncogene, as identified by gene expression profiling, is essential for the transformation of endothelial cells. J Virol. 2002;76: 8383-8399.
Lawson ND, Vogel AM, Weinstein BM. Sonic hedgehog and vascular endothelial growth factor act upstream of the notch pathway during arterial endothelial differentiation. Dev Cell. 2002;3: 127-136.
Albini A, Soldi R, Giunciuglio D, et al. The angiogenesis induced by HIV-1 tat protein is mediated by the Flk-1/KDR receptor on vascular endothelial cells. Nat Med. 1996;2: 1371-1375.
Barillari G, Sgadari C, Palladino C, et al. Inflammatory cytokines synergize with the HIV-1 Tat protein to promote angiogenesis and Kaposi's sarcoma via induction of basic fibroblast growth factor and the alpha v beta 3 integrin. J Immunol. 1999;163: 1929-1935.
Curtin JF, Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003;15: 983-992.
Erdman KS. The protein tyrosine phosphatase PTP-basophil/basophil-like: interacting proteins and molecular functions. Eur J Biochem. 2003; 270: 4789-4798.(Rizwan Masood, Guangbin X)