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Amino Acid Preferences for a Critical Substrate Bi
http://www.100md.com 病菌学杂志 2005年第7期
     Department of Biochemistry and Molecular Biology, Research Center for Molecular Medicine, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary

    ABSTRACT

    The specificities of the proteases of 11 retroviruses representing each of the seven genera of the family Retroviridae were studied using a series of oligopeptides with amino acid substitutions in the P2 position of a naturally occurring type 1 cleavage site (Val-Ser-Gln-Asn-TyrPro-Ile-Val-Gln; the arrow indicates the site of cleavage) in human immunodeficiency virus type 1 (HIV-1). This position was previously found to be one of the most critical in determining the substrate specificity differences of retroviral proteases. Specificities at this position were compared for HIV-1, HIV-2, equine infectious anemia virus, avian myeloblastosis virus, Mason-Pfizer monkey virus, mouse mammary tumor virus, Moloney murine leukemia virus, human T-cell leukemia virus type 1, bovine leukemia virus, human foamy virus, and walleye dermal sarcoma virus proteases. Three types of P2 preferences were observed: a subgroup of proteases preferred small hydrophobic side chains (Ala and Cys), and another subgroup preferred large hydrophobic residues (Ile and Leu), while the protease of HIV-1 preferred an Asn residue. The specificity distinctions among the proteases correlated well with the phylogenetic tree of retroviruses prepared solely based on the protease sequences. Molecular models for all of the proteases studied were built, and they were used to interpret the results. While size complementarities appear to be the main specificity-determining features of the S2 subsite of retroviral proteases, electrostatic contributions may play a role only in the case of HIV proteases. In most cases the P2 residues of naturally occurring type 1 cleavage site sequences of the studied proteases agreed well with the observed P2 preferences.

    INTRODUCTION

    The retroviral proteases (PR), especially those of human immunodeficiency viruses (HIV), have received a great deal of attention as a target for chemotherapy (for reviews, see references 20 and 39), since the protease activity is required for the maturation and infectivity of the viruses (reviewed in references 16 and 24). Specificity studies of wild-type and mutant HIV PRs have provided a basis for the rational design of potent, selective inhibitors (6, 32, 46) and also may help to circumvent the problems caused by the rapidly developing resistance against the compounds used therapeutically (30).

    Another experimental approach to better understand PR specificity is to characterize the PRs of divergent retroviruses, since several of the mutations causing drug resistance in HIV type 1 (HIV-1) PR introduce residues into the substrate binding sites found in equivalent positions in other retroviruses (15, 27). Previously, a large series of peptides containing single-amino-acid substitutions in the P4-P3' (nomenclature according to reference 29) region of the Val-Ser-Gln-Asn-TyrPro-Ile-Val-Gln oligopeptide (the arrow indicates the site of cleavage) were used to characterize the specificities of the proteases of various retroviruses, including those of HIV-1, HIV-2 (38, 40), equine infectious anemia virus (EIAV) (44), Moloney murine leukemia virus (MMLV) (22), and avian myeloblastosis virus (AMV) (34). Activities relative to the unmodified peptide were determined for substituted peptides (except for MMLV PR) to compare the specificities of the enzymes. Based on these studies, the P2 position was found to be one of the most critical in determining the substrate specificity differences of retroviral proteases. Here, we report an extension of these studies of the P2 position with a protease set which now contains at least one member of each genus of the family Retroviridae (Table 1), including HIV-1, HIV-2, EIAV, MMLV, AMV, Mason-Pfizer monkey virus (MPMV), mouse mammary tumor virus (MMTV), human T-cell leukemia virus type 1 (HTLV-1), bovine leukemia virus (BLV), human foamy virus (HFV), and walleye dermal sarcoma virus (WDSV) proteases. Our present study has the advantage that the different retroviral proteases were mapped with the same peptide series under the same reaction conditions in the same laboratory, and therefore, the results are directly comparable. Similarities and differences in the P2 specificities of retroviral proteases were interpreted by molecular modeling using the crystal structures and homologous models of retroviral proteases.

    MATERIALS AND METHODS

    Retroviral proteases. Chemically synthesized HIV-2 protease was purified and refolded as described previously (40). Purified MPMV protease (the shortest, 12-kDa, form) was prepared as described previously (48). MMTV protease was expressed as a glutathione S-transferase fusion protein, processed with factor Xa, and purified following the published procedure (23). MMLV protease was cloned with maltose binding protein (MBP) and hexahistidine tags and purified as described previously (7). HTLV-1 protease (15), BLV protease (49), and WDSV protease (11) were purified from inclusion bodies after expression as described previously. HFV protease was cloned in fusion with MBP and used in its fusion form (10).

    Oligopeptides. Oligopeptides synthesized by solid-phase peptide synthesis were described previously (21, 23, 40). Stock solutions and dilutions were made in distilled water (or in 5 mM dithiothreitol for the Cys-containing peptide), and the proper peptide concentrations were determined by amino acid analysis.

    Protease assay. Protease assays were performed at 37°C using purified retroviral proteases and chemically synthesized oligopeptides (0.4 mM) in 0.25 M potassium phosphate buffer, pH 5.6, containing 7.5% glycerol, 5 mM dithiothreitol, 1 mM EDTA, 0.2% Nonidet P-40, and 2 M NaCl. The reaction mixtures were incubated at 37°C for 1 h, or 24 h in the cases of HFV and WDSV proteases, and the reactions were stopped by the addition of 9 volumes of 1% trifluoroacetic acid. The reaction mixtures were then injected onto a Nova-Pak C18 reversed-phase chromatography column (3.9 x 150 mm; Waters Associates, Inc.) using an automatic injector. The substrates and the cleavage products were separated using an acetonitrile gradient (0 to 100%) in water in the presence of 0.05% trifluoroacetic acid. The cleavage of peptides was monitored at 206 nm, and the peak areas were integrated. Amino acid analysis of the collected peaks was used to confirm the site of cleavage with HIV-1 PR (40). For the other retroviral proteases, the cleavage products were identified by the retention time, which was found to be identical to that obtained with HIV-1 PR. Relative activities were calculated from the molar amounts of peptides cleaved per unit time at <20% substrate turnover by dividing the activity on a given peptide by the activity on the Val-Ser-Gln-Val-TyrPro-Ile-Val-Gln substrate, which has the smallest hydrocarbon side chain at the P2 site among the peptides that were hydrolyzable by all of the studied proteases, as described in reference 1. Measurements were performed in duplicate, and the average values were calculated. The standard error was <10%. The relative activities for the HIV-1 PR (40), EIAV PR (44), and AMV PR (34) have been reported previously. The published values were converted to values relative to our reference substrate. Previous studies also indicated a strong correlation between the relative activities and the specificity constants (34); therefore, the determined activity values can be considered a measure of the kcat/Km values.

    For peptides representing naturally occurring cleavage sites, at least six substrate concentrations were tested, selected based on the approximate Km values. Amino acid analysis of the collected product peaks was used to confirm the sites of cleavages and to establish the relationship between integration values and product quantities. Kinetic parameters were determined by fitting the data obtained at <20% substrate hydrolysis to the Michaelis-Menten equation by using the Fig. P program (Fig. P Software Corp.). The standard errors of the kinetic parameters were <20%.

    Molecular modeling. Structure-based alignment of the HIV-1, HIV-2, simian immunodeficiency virus, EIAV, feline immunodeficiency virus (FIV), and Rous sarcoma virus (RSV) PRs was used as a template for the alignment of BLV, HTLV-1, MPMV, MMTV, MMLV, HFV, and WDSV protease sequences, as shown in Fig. 1. Structural alignment was done by WHAT IF (43), and the initial multiple sequence alignment was made by ClustalW (31) and was corrected by hand based on the structural alignment. The phylogenetic tree was made by ClustalW and Phylip (9).

    The program Modeller (28) was used to build the initial models of all studied retroviral proteases. The program allows the use of multiple template crystal structures as an input and can create multiple homologous models as an output. We used the highest-resolution (1.2- to 2.4-?) crystal structures of HIV-1 (18), HIV-2 (33), EIAV (13), FIV (14), and RSV (47) proteases (either as wild-type or mutant forms) available at the time of modeling as templates (Protein Data Bank accession codes [and resolutions]: 1K1T [1.2 ?], 1IDA [1.7 ?], 1FMB [1.8 ?], 4FIV [1.8 ?], and 1BAI [2.4 ?], respectively) and generated three simultaneous models for each protease. RSV protease structure was used to interpret the AMV protease results: AMV and RSV proteases differ in only two residues, which are not expected to be involved in the enzyme-substrate interactions (34). A model of the QVYPIV hexapeptide was docked into the substrate binding site of each retroviral protease model in forward and reverse directions, and a water molecule was also placed in a conserved position between the flaps of the protease and the substrate. This sequence has the smallest hydrocarbon side chain at the P2 site among the peptides that were hydrolyzable by all of the studied proteases, and the appropriate substrate was also used as a reference substrate for activity measurements.

    Unfavorable van der Waals interactions were removed by short minimizations using Sybyl (Tripos Inc., St. Louis, Mo.) with the following parameters: Kollman all-atom force field (45) as implemented in Sybyl, 8 ? cutoff; 20 Simplex and 100 Powell iterations with distance constraints between the carboxyl groups of the two catalytic aspartates and between the H bond donor and acceptor atoms of the enzyme and the substrate in the P3-P3' region to maintain the conserved H bond network of the substrate binding site. Another 100 Powell iterations were applied with distance constraints only between the catalytic aspartates.

    The structures were examined on a Silicon Graphics Indigo2 or O2 computer graphics system using the program Sybyl or WHAT IF. Root mean square (RMS) deviations were calculated by the structural superposition module of WHAT IF. Cavities were calculated on the minimized structures containing P2 Gly substrate using the SiteID module of Sybyl, and they were averaged on three models and two directions for each enzyme. At least three values were included in the averaging procedure for each enzyme. The volume of the amino acid residues was retrieved from the literature (50). The residues forming the S2 subsites (Fig. 1) were predicted previously for HIV-1 and HIV-2 PRs (40); EIAV (44), AMV (34), MMLV (2), and HTLV-1 (41) proteases and the corresponding residues in BLV, MMTV, MPMV, HFV, and WDSV proteases were obtained from the sequence alignment in Fig. 1 and verified using the crystal structures and homologous models.

    RESULTS AND DISCUSSION

    It is a special feature of the retroviral proteases that it is not possible to give consensus substrate sequences for them, even though they are fairly specific. Retroviral protease cleavage sites are presently classified into two groups. Type 1 cleavage sites have the aromatic residue Pro, and type 2 sites have hydrophobic residues (excluding Pro) at the site of cleavage (12, 25, 40). The P2 position is also critical in determining the type of cleavage site (12, 40). In type 1 cleavage sites of primate lentiviruses, like HIV-1, there is a preference for Asn at P2, while in type 2 cleavage sites, the P2 position is typically ? branched. The type 1 cleavage site is very important for several reasons. No protease except pepsin is known to act at the imino side of a Pro residue. Proline residues, especially after Tyr or Phe in the sequence (as is the case in type 1 cleavage sites), have a relatively high probability of forming the cis isomer rather than the trans isomer of the preceding peptide bond (17). Conformational selectivity of the HIV-1 PR toward the trans isomer of the cleaved peptide bond was demonstrated by nuclear magnetic resonance and kinetic studies (19, 42).

    Previously, we characterized the specificities of HIV-1, HIV-2, EIAV, and AMV proteases using an oligopeptide substrate series based on the naturally occurring type 1 cleavage site (Val-Ser-Gln-Asn-TyrPro-Ile-Val-Gln) between the matrix (MA) and capsid (CA) proteins of HIV-1 (34, 40, 44). In this study, we extended these studies to the proteases of MPMV, MMTV, MMLV, HTLV-1, BLV, HFV, and WDSV to have at least one member of each genus of the retroviruses (Table 1).

    Replacement of the P2 Asn in the original peptide with medium-size hydrophobic residues resulted in peptides that were well hydrolyzed by most proteases (Table 1). The largest effects were observed with HTLV-1 and HFV proteases, in which the original nonhydrolyzable peptide was converted to hydrolyzable ones, and with BLV PR, in which the best substrate was the P2 Leu-containing peptide, which was an almost 200-fold better substrate than the unmodified peptide. P2 Leu-containing peptide was also the best substrate for MMLV PR, as described in a previous study (22). The peptide with an Ile substitution was the best substrate for MMTV and HTLV-1 proteases. The small hydrophobic amino acid Ala was preferred by AMV and MPMV proteases. EIAV PR preferred the slightly polar Cys residue, while HIV-1 PR preferred the more polar Asn residue, giving the best value with the original peptide (Table 1). Although in the cases of HIV-2 and WDSV proteases, Cys was the best residue in this position, a similar value was obtained for Asn (Table 1). HFV PR showed equally high activities, within the experimental error of the measurement, on substrates containing Ala, Val, or Cys at the P2 site. Therefore, in this sequence context, the preference for Asn by the HIV-1 PR is rather exceptional, since the other retroviral proteases studied showed higher preferences for hydrophobic or less polar residues. It seems to be a common result for retroviral proteases that Phe or Gly at P2 of this peptide series forms a poor substrate: molecular modeling suggested that the Phe side chain is too bulky, while Gly is too small, for this subsite.

    To understand the similarities and differences among the specificities of retroviral proteases, molecular models were built for all of the studied enzymes using the same procedure. The goodness of the models was estimated by calculating their RMS deviations from the five high-resolution crystal structures used as templates. Naturally, the smallest values (0.3 to 0.5 ?) were calculated for HIV-1, HIV-2, EIAV, and RSV models from their own crystal structure templates, and their range showed similar values, as can be seen in comparison to different crystallographic determinations of the same enzyme (4). Other models showed similar RMS deviations (0.9 to 2.9 ?) from the values calculated between the templates themselves (1.0 to 2.7 ?), and they also showed similar relationships between the RMS deviation and the sequence homology, as can be seen in a comparison of crystal structures (4).

    Based on the molecular models, the S2 binding sites of retroviral proteases are usually small hydrophobic pockets. S2 is sterically more restricted than the other subsites, especially S4 and S3. About half of the subsite-forming residues of S2 are conserved (Fig. 1), however, in place of Asp 30 of HIV-1 PR and HIV-2 PR, which appears to be the reason why these enzymes could accommodate more polar residues in this subsite (3, 40); other enzymes contain hydrophobic or hydrophilic residues, but pointing outward from the pocket. At the tip of the flap, most enzymes contain branched side chains (Ile, Val, or Leu), but MMLV, BLV, and HTLV-1 PRs contain smaller Ala residues, which may be at least partly responsible for the greater Ile and Leu preference at the S2 sites of these enzymes. Previously, we observed that the two residues that seemed to be crucial in determining the preference for Val over Leu at P2 in our substrate series were residues of the retroviral proteases corresponding to Val 32 and Ile 47 of HIV-1 (34): in both positions, Ile favors Val at P2 in the substrate, while Val favors Leu. BLV, HTLV-1, and MMLV PRs have Val at both positions, and the relative preference for Leu over Val is in good agreement with the previously proposed rule (34). This suggests that size complementarities are the main specificity-determining features of the S2 subsites of retroviral PRs, and electrostatic contributions play a role only in the cases of proteases of primate lentiviruses (HIVs and simian immunodeficiency viruses). To illustrate this suggestion, cavity volumes were calculated in the cases of P2 Gly substrate complexes and plotted against the averaged volume of the best two P2 residues measured experimentally (Fig. 2). The data points (except that of HIV-1 PR) showed linear correlations (r = 0.96) and were clustered into two regions of the plot according to the P2 preferences of the enzymes. This correlation was further supported by phylogenetic analysis of the sequences of retroviral proteases (Fig. 3).

    Naturally occurring type 1 cleavage site sequences of the studied retroviruses (Table 2) have P2 residues that are usually in good agreement with the findings of the S2 mapping study (Table 1). Asn can be found at the P2 position in five out of six cleavage sites of HIV-1 and HIV-2, and the peptide with P2 Leu showed a very low cleavage efficiency. The S2 specificity of EIAV PR appeared to show a transition between the specificity of primate lentiviruses (preference for polar residues) and the alpha-, beta-, and gammaretroviruses (preference for small hydrophobic residues): the preferred residues are Cys and Ala in the context of the VSQXYPIVQ sequence, while Glu and Thr are found in the natural cleavage sites of EIAV. The best P2 residue (Ala) observed for AMV protease in the mapping study appears in the P2 position of its naturally occurring type 1 cleavage site sequence; however, the MPMV and MMTV sites contain the less favorable Ile and Thr, respectively, at this position (Table 2). In one of the MMLV cleavage sites, the most preferred Leu appears in P2, while the other contains a less preferred Ala. Discrepancies between the most preferred P2 residues and those observed in the naturally occurring cleavage sites might be due to the different sequence contexts, which have been shown to have a profound effect on the subsite preference of HIV-1 PR, including S2 (35), as well as to the possibility that not all retroviral cleavage sites are optimized evolutionarily for rapid processing (8).

    The importance of the proper van der Waals interactions and filling in the S2 binding site is also demonstrated by mutations appearing in drug resistance. One of the slowest HIV-1 cleavage sites, the NC/p1 cleavage site (Arg-Gln-Ala-AsnPhe-Leu-Gly-Lys) contains a small Ala residue at P2, which does not fit optimally into this pocket, as demonstrated by kinetic, modeling (8), and crystallographic studies (26); this residue is frequently mutated to Val in drug resistance (5) to provide a substantially better fit.

    One of the main problems in the protease inhibitor therapy of AIDS is the development of resistance to the drugs designed against HIV-1 PR. Many of the mutations occurring in drug resistance produce residues that can be found in other retroviral proteases. Therefore, understanding the specificity similarities and differences of these enzymes may help in the design of broad-spectrum inhibitors against HIV-1 PR.

    ACKNOWLEDGMENTS

    This research was supported by the Hungarian Science and Research Fund (OTKA T22670, T43482, and F34479) and by the János Bolyai Fellowship of the Hungarian Academy of Sciences (to Péter Bagossi).

    We thank Stephen Oroszlan for providing the MMTV protease clone, John M. Louis for the HIV-1 protease clone, Iva Pichova for the MPMV protease clone, and Volker M. Vogt for the WDSV protease clone. Ivo Bláha is acknowledged for the synthesis of the peptides. We thank Szilvia Pet? for technical assistance.

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