Efficient isolation of targeted Caenorhabditis elegans deletion strain
http://www.100md.com
《核酸研究医学期刊》
The authors would like to apologize for failing to correct an error in their description of the two-step PCR used in their protocol, which reverses the order of the primer pairs used for the first and second rounds of PCR. This error is found in two locations in the manuscript:
On page 2, ‘Two rounds of amplification were used, an initial round of 35 cycles with the inner primer set, followed by a second round of 35 cycles with the corresponding outer primer set.’ should be changed to ‘Two rounds of amplification were used, an initial round of 35 cycles with the outer primer set, followed by a second round of 35 cycles with the corresponding inner primer set.’
On page 3, in the legend for Figure 1, ‘Second round PCRs (outer primers) were performed with a dilution of the first round (inner primers) reaction.’ should be changed to ‘Second round PCRs (inner primers) were performed with a dilution of the first round (outer primers) reaction.’
The correct order of primer sets that they used for two-step PCR is outer primer set for the first round, followed by inner primer set for the second round.
This descriptive error does not alter any of the conclusions of the paper. The authors regret any inconvenience or misunderstanding that their error may have caused.(Aguan Wei, Alex Yuan, Gloria Fawcett, Al)
On page 2, ‘Two rounds of amplification were used, an initial round of 35 cycles with the inner primer set, followed by a second round of 35 cycles with the corresponding outer primer set.’ should be changed to ‘Two rounds of amplification were used, an initial round of 35 cycles with the outer primer set, followed by a second round of 35 cycles with the corresponding inner primer set.’
On page 3, in the legend for Figure 1, ‘Second round PCRs (outer primers) were performed with a dilution of the first round (inner primers) reaction.’ should be changed to ‘Second round PCRs (inner primers) were performed with a dilution of the first round (outer primers) reaction.’
The correct order of primer sets that they used for two-step PCR is outer primer set for the first round, followed by inner primer set for the second round.
This descriptive error does not alter any of the conclusions of the paper. The authors regret any inconvenience or misunderstanding that their error may have caused.(Aguan Wei, Alex Yuan, Gloria Fawcett, Al)