Widely Distributed and Predominant CTX-M Extended-Spectrum -Lactamases
http://www.100md.com
《微生物临床杂志》
Academic Medical Center
Onze Lieve Vrouwe Gasthuis
Vrije Universiteit Medical Center
Slotervaartziekenhuis
Sint Lucas Andreas Ziekenhuis, Amsterdam, The Netherlands
ABSTRACT
Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX-M ESBLs dominated, and four types were identified in 18 isolates.
TEXT
Extended-spectrum beta-lactamases (ESBLs) are of growing concern. More than 200 different natural ESBL variants are known at present, and their evolution has been so fast that a website (http://www.lahey.org/studies) was initiated to report new progress in this field (18). While ESBLs were initially found mainly in Klebsiella pneumoniae and Escherichia coli, an increasing variety of gram-negative species have been shown to harbor such resistance mechanisms (7, 8). In recent years, ESBLs have become more prevalent in AmpC-producing species (1, 16). In spite of the worldwide use of -lactam antimicrobial agents, which is considered the main reason for the emergence of ESBLs (2), the distributions of ESBLs are far from uniform. There are considerable geographical differences in prevalence of ESBLs in Europe; in general, the prevalence is lower in northern European countries than in eastern and southern European countries (5). In a French multicenter study (1996 to 2000), the prevalence of ESBLs was found to be 11.4% in K. pneumoniae isolates and 47.7% in Enterobacter aerogenes isolates (1). The prevalence of ESBL-producing E. coli and Klebsiella spp. isolates in Dutch hospitals was less than 1% in 1997 (17).
(Some of the information in this report was presented at the annual meeting of the NVMM/NVvM, April 2005.)
The aim of the present study was to evaluate the prevalence and the molecular epidemiology of ESBLs in Amsterdam, The Netherlands. During one week in 2004, five microbiological laboratories of five hospitals (Academic Medical Center [AMC, 1,002 beds], Vrije Universiteit Medical Center [VUMC, 733 beds], Onze Lieve Vrouwe Gasthuis [OLVG, 530 beds], Slotervaartziekenhuis [SLZ, 490 beds], and Sint Lucas Andreas Ziekenhuis [SLAZ, 551 beds]) in Amsterdam participated. Enterobacteriaceae and nonfermenting gram-negative bacilli cultured from clinical specimens were obtained, and one isolate per species per patient was included in the study. Three hundred sixty strains were collected, comprising 14 species (Table 1) : 144 E. coli strains, 47 Klebsiella spp. strains, 94 non-E. coli non-Klebsiella Enterobacteriaceae, and 75 nonfermenters (Table 1). The following numbers of strains were isolated from each hospital: 123 from AMC, 70 from VUMC, 78 from OLVG, 63 from SLZ, and 26 from SLAZ. The strains were considered nonclonal according to the information from the participating hospitals, the antimicrobial susceptibility pattern, and common biochemical reactions.
Screening for ESBL production was performed with the cefpodoxime disk diffusion method (3). When a bacterial strain showed a zone diameter of 20 mm for cefpodoxime, we considered the isolate a suspected ESBL producer. The phenotypic confirmation for ESBL production was determined with a double and combined disk test (N. al Naiemi et al., submitted for publication), which includes disks of ceftazidime, cefotaxime, cefpodoxime, and cefepime placed around a disk containing amoxicillin plus clavulanate. A disk of ceftazidime plus clavulanate was also included. Susceptibility tests for gentamicin, tobramycin, ciprofloxacin, cotrimoxazole, imipenem, and piperacillin plus tazobactam were performed with Neo-Sensitabs from Rosco (Taastrup, Denmark), according to CLSI guidelines (3).
Fifty-two strains showed decreased susceptibility for cefpodoxime. Of these strains, 28 produced ESBLs and showed decreased susceptibility to several other antimicrobial agents (Table 2). The prevalence of ESBL-producing strains was 7.8% (Table 1). The prevalence for each hospital was as follows: 9.8% (12/123) for AMC, 7.1% (5/70) for VUMC, 6.4% (5/78) for OLVG, 6.3% (4/63) for SLZ, and 7.7% (2/26) for SLAZ.
The presence of blaSHV, blaTEM, and blaCTX-M genes was confirmed by PCR and sequence analysis as described in a previous study (9). To prevent carryover contamination, the uracil system was applied. Well-characterized plasmid DNA was used as a positive control and ultra-high-purity water as a negative control. Eighteen isolates contained blaCTX-M and 10 isolates contained blaSHV ESBL genes. No blaTEM ESBL genes were detected (Table 2).
The present survey shows that the overall prevalence of ESBL-producing gram-negative organisms in hospitals in the Amsterdam region in 2004 was still below 10% but was much higher than the prevalence as determined in a nationwide survey in 1997. The previous survey was performed in eight university hospitals, including the Academic Medical Center (AMC) which participated in the current study, and three large regional laboratories and showed that the prevalence of ESBL among isolates of E. coli and Klebsiella spp. was <1% (17). During the 1997 study, no ESBLs were found in the AMC. However, ESBL-producing Enterobacteriaceae, including E. coli and Klebsiella spp. organisms, have been isolated on several occasions during the past 5 years (9). In this study, the highest prevalence of ESBL producers, which amounted to 15%, was observed with non-E. coli non-Klebsiella Enterobacteriaceae (Table 1). This is of concern, since spread of resistance among these species is known to occur through plasmids and these species are therefore particularly prone to spread and cause outbreaks (4, 9, 10, 11, 15). To date, SHV-type ESBLs dominated in surveys of resistant clinical isolates in Europe and North America (12, 20). This was also the case in one of the participating hospitals in Amsterdam (AMC) in 2000 (9). In 2003, CTX-M ESBL genes were detected in the AMC for the first time, and the present study shows the dissemination of four different CTX-M ESBLs in nine different species, including the nonfermenting gram-negative species (Table 1). This wide occurrence is remarkable. Pitout et al. (14) reported the spread of Enterobacteriaceae carrying different CTX-M ESBLs, originating from the community. Possibly, a similar phenomenon is occurring in Amsterdam. However, we cannot exclude the possibility of the spread of these different groups of CTX-M enzymes between the medical centers or through other routes, e.g., patient transfer.
The emergence and the increase in the prevalence of CTX-M ESBLs is remarkable and may be correlated with the increased use of cefotaxime, which has been suggested to play an important role in the selection for CTX-M ESBLs (13, 19). The gentamicin resistance rate in ESBL producers in this study was high compared to that found among all gram-negative isolates from patients in Dutch hospitals (6) and confirms that the use of non--lactam antibiotics for treatment of infections caused by ESBL-producing pathogens is limited and that only a few agents, such as carbapenems, provide solid coverage. More rational analysis of antibiotic policies and of risk factors for carriage of ESBL-producing pathogens among patients is required.
The present study suggests that ESBLs are a growing problem in Amsterdam and that the establishment of these baseline data is crucial for the evaluation of the epidemiology of ESBLs and the antibiotic policy in this region.
ACKNOWLEDGMENTS
N. al Naiemi participated in the phenotypic and molecular typing of strains and genes. N. al Naiemi, A. Bart, M. D. de Jong, C. M. Vandenbroucke-Grauls, and B. Duim participated in the development of a research strategy, directed the analyses, and led the writing of the manuscript.
P. C. Wever, Y. J. Debets-Ossenkopp, P. J. G. M. Rietra, L. Spanjaard, and A. J. Bos participated in collection and identification of the bacterial isolates. All authors appraised and approved the final report.
The authors do not have a commercial or other association that might pose a conflict of interest. This study was performed without external financial support.
FOOTNOTES
Corresponding author. Mailing address: Dept. of Medical Microbiology, Academic Medical Center, L1-244, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Phone: 31 20 5665714. Fax: 31 20 5669745. E-mail: b.duim@amc.uva.nl.
Current address: Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
REFERENCES
Albertini, M. T., C. Benoit, L. Berardi, Y. Berrouane, A. Boisivon, P. Cahen, C. Cattoen, Y. Costa, P. Darchis, E. Deliere, D. Demontrond, F. Eb, F. Golliot, G. Grise, A. Harel, J. L. Koeck, M. P. Lepennec, C. Malbrunot, M. Marcollin, S. Maugat, M. Nouvellon, B. Pangon, S. Ricouart, M. Roussel-Delvallez, A. Vachee, A. Carbonne, L. Marty, V. Jarlier, et al. 2002. Surveillance of methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBLE) in Northern France: a five-year multicentre incidence study. J. Hosp. Infect. 52:107-113.
Bradford, P. A. 2001. Extended-spectrum -lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14:933-951.
Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement (M100-S15). Clinical and Laboratory Standards Institute, Wayne, Pa.
Dumarche, P., C. De Champs, D. Sirot, C. Chanal, R. Bonnet, and J. Sirot. 2002. TEM derivative-producing Enterobacter aerogenes strains: dissemination of a prevalent clone. Antimicrob. Agents Chemother. 46:1128-1131.
Goossens, H. 2001. MYSTIC program: summary of European data from 1997 to 2000. Diagn. Microbiol. Infect. Dis. 41:183-189.
Kaiser, A. M., C. Schultsz, G. J. Kruithof, Y. Debets-Ossenkopp, and C. Vandenbroucke-Grauls. 2004. Carriage of resistant microorganisms in repatriates from foreign hospitals to The Netherlands. Clin. Microbiol. Infect. 10:972-979.
Marchandin, H., C. Carriere, D. Sirot, H. J. Pierre, and H. Darbas. 1999. TEM-24 produced by four different species of Enterobacteriaceae, including Providencia rettgeri, in a single patient. Antimicrob. Agents Chemother. 43:2069-2073.
Naas, T., L. Philippon, L. Poirel, E. Ronco, and P. Nordmann. 1999. An SHV-derived extended-spectrum -lactamase in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 43:1281-1284.
Naiemi, N. A., B. Duim, P. H. Savelkoul, L. Spanjaard, E. de Jonge, A. Bart, C. M. Vandenbroucke-Grauls, and M. D. de Jong. 2005. Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology. J. Clin. Microbiol. 43:4862-4864.
Neuwirth, C., E. Siebor, J. Lopez, A. Pechinot, and A. Kazmierczak. 1996. Outbreak of TEM-24-producing Enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family Enterobacteriaceae. J. Clin. Microbiol. 34:76-79.
Palucha, A., B. Mikiewicz, W. Hryniewicz, and M. Gniadkowski. 1999. Concurrent outbreaks of extended-spectrum beta-lactamase-producing organisms of the family Enterobacteriaceae in a Warsaw hospital. J. Antimicrob. Chemother. 44:489-499.
Paterson, D. L., K. M. Hujer, A. M. Hujer, B. Yeiser, M. D. Bonomo, L. B. Rice, R. A. Bonomo, and the International Klebsiella Study Group. 2003. Extended-spectrum -lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type -lactamases. Antimicrob. Agents Chemother. 47:3554-3560.
Patterson, J. E. 2001. Antibiotic utilization: is there an effect on antimicrobial resistance Chest 119:426S-430S.
Pitout, J. D., D. B. Gregson, D. L. Church, S. Elsayed, and K. B. Laupland. 2005. Community-wide outbreaks of clonally related CTX-M-14 -lactamase-producing Escherichia coli strains in the Calgary health region. J. Clin. Microbiol. 43:2844-2849.
Pitout, J. D., K. S. Thomson, N. D. Hanson, A. F. Ehrhardt, P. Coudron, and C. C. Sanders. 1998. Plasmid-mediated resistance to expanded-spectrum cephalosporins among Enterobacter aerogenes strains. Antimicrob. Agents Chemother. 42:596-600.
Sirot, J., M. H. Nicolas-Chanoine, H. Chardon, J. L. Avril, C. Cattoen, J. C. Croix, H. Dabernat, T. Fosse, J. C. Ghnassia, E. Lecaillon, A. Marmonier, M. Roussel-Delvallez, C. J. Soussy, A. Trevoux, F. Vandenesch, C. Dib, N. Moniot-Ville, and Y. Rezvani. 2002. Susceptibility of Enterobacteriaceae to beta-lactam agents and fluoroquinolones: a 3-year survey in France. Clin. Microbiol. Infect. 8:207-213.
Stobberingh, E. E., J. Arends, J. A. Hoogkamp-Korstanje, W. H. Goessens, M. R. Visser, A. G. Buiting, Y. J. Debets-Ossenkopp, R. J. van Ketel, M. L. van Ogtrop, L. J. Sabbe, G. P. Voorn, H. L. Winter, and J. H. van Zeijl. 1999. Occurrence of extended-spectrum betalactamases (ESBL) in Dutch hospitals. Infection 27:348-354.
Sturenburg, E., and D. Mack. 2003. Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control. J. Infect. 47:273-295.
Wang, H., S. Kelkar, W. Wu, M. Chen, and J. P. Quinn. 2003. Clinical isolates of Enterobacteriaceae producing extended-spectrum -lactamases: prevalence of CTX-M-3 at a hospital in China. Antimicrob. Agents Chemother. 47:790-793.
Yuan, M., H. Aucken, L. M. Hall, T. L. Pitt, and D. M. Livermore. 1998. Epidemiological typing of klebsiellae with extended-spectrum beta-lactamases from European intensive care units. J. Antimicrob. Chemother. 41:527-539.(N. al Naiemi, A. Bart, M. D. de Jong, C.)
Onze Lieve Vrouwe Gasthuis
Vrije Universiteit Medical Center
Slotervaartziekenhuis
Sint Lucas Andreas Ziekenhuis, Amsterdam, The Netherlands
ABSTRACT
Three hundred sixty Enterobacteriaceae and nonfermenting gram-negative bacilli, isolated during one week in May 2004 at five hospitals in Amsterdam, The Netherlands, were evaluated for the presence of extended-spectrum beta-lactamases (ESBLs). A prevalence of 7.8% was found, in contrast to the 1% observed in 1997. CTX-M ESBLs dominated, and four types were identified in 18 isolates.
TEXT
Extended-spectrum beta-lactamases (ESBLs) are of growing concern. More than 200 different natural ESBL variants are known at present, and their evolution has been so fast that a website (http://www.lahey.org/studies) was initiated to report new progress in this field (18). While ESBLs were initially found mainly in Klebsiella pneumoniae and Escherichia coli, an increasing variety of gram-negative species have been shown to harbor such resistance mechanisms (7, 8). In recent years, ESBLs have become more prevalent in AmpC-producing species (1, 16). In spite of the worldwide use of -lactam antimicrobial agents, which is considered the main reason for the emergence of ESBLs (2), the distributions of ESBLs are far from uniform. There are considerable geographical differences in prevalence of ESBLs in Europe; in general, the prevalence is lower in northern European countries than in eastern and southern European countries (5). In a French multicenter study (1996 to 2000), the prevalence of ESBLs was found to be 11.4% in K. pneumoniae isolates and 47.7% in Enterobacter aerogenes isolates (1). The prevalence of ESBL-producing E. coli and Klebsiella spp. isolates in Dutch hospitals was less than 1% in 1997 (17).
(Some of the information in this report was presented at the annual meeting of the NVMM/NVvM, April 2005.)
The aim of the present study was to evaluate the prevalence and the molecular epidemiology of ESBLs in Amsterdam, The Netherlands. During one week in 2004, five microbiological laboratories of five hospitals (Academic Medical Center [AMC, 1,002 beds], Vrije Universiteit Medical Center [VUMC, 733 beds], Onze Lieve Vrouwe Gasthuis [OLVG, 530 beds], Slotervaartziekenhuis [SLZ, 490 beds], and Sint Lucas Andreas Ziekenhuis [SLAZ, 551 beds]) in Amsterdam participated. Enterobacteriaceae and nonfermenting gram-negative bacilli cultured from clinical specimens were obtained, and one isolate per species per patient was included in the study. Three hundred sixty strains were collected, comprising 14 species (Table 1) : 144 E. coli strains, 47 Klebsiella spp. strains, 94 non-E. coli non-Klebsiella Enterobacteriaceae, and 75 nonfermenters (Table 1). The following numbers of strains were isolated from each hospital: 123 from AMC, 70 from VUMC, 78 from OLVG, 63 from SLZ, and 26 from SLAZ. The strains were considered nonclonal according to the information from the participating hospitals, the antimicrobial susceptibility pattern, and common biochemical reactions.
Screening for ESBL production was performed with the cefpodoxime disk diffusion method (3). When a bacterial strain showed a zone diameter of 20 mm for cefpodoxime, we considered the isolate a suspected ESBL producer. The phenotypic confirmation for ESBL production was determined with a double and combined disk test (N. al Naiemi et al., submitted for publication), which includes disks of ceftazidime, cefotaxime, cefpodoxime, and cefepime placed around a disk containing amoxicillin plus clavulanate. A disk of ceftazidime plus clavulanate was also included. Susceptibility tests for gentamicin, tobramycin, ciprofloxacin, cotrimoxazole, imipenem, and piperacillin plus tazobactam were performed with Neo-Sensitabs from Rosco (Taastrup, Denmark), according to CLSI guidelines (3).
Fifty-two strains showed decreased susceptibility for cefpodoxime. Of these strains, 28 produced ESBLs and showed decreased susceptibility to several other antimicrobial agents (Table 2). The prevalence of ESBL-producing strains was 7.8% (Table 1). The prevalence for each hospital was as follows: 9.8% (12/123) for AMC, 7.1% (5/70) for VUMC, 6.4% (5/78) for OLVG, 6.3% (4/63) for SLZ, and 7.7% (2/26) for SLAZ.
The presence of blaSHV, blaTEM, and blaCTX-M genes was confirmed by PCR and sequence analysis as described in a previous study (9). To prevent carryover contamination, the uracil system was applied. Well-characterized plasmid DNA was used as a positive control and ultra-high-purity water as a negative control. Eighteen isolates contained blaCTX-M and 10 isolates contained blaSHV ESBL genes. No blaTEM ESBL genes were detected (Table 2).
The present survey shows that the overall prevalence of ESBL-producing gram-negative organisms in hospitals in the Amsterdam region in 2004 was still below 10% but was much higher than the prevalence as determined in a nationwide survey in 1997. The previous survey was performed in eight university hospitals, including the Academic Medical Center (AMC) which participated in the current study, and three large regional laboratories and showed that the prevalence of ESBL among isolates of E. coli and Klebsiella spp. was <1% (17). During the 1997 study, no ESBLs were found in the AMC. However, ESBL-producing Enterobacteriaceae, including E. coli and Klebsiella spp. organisms, have been isolated on several occasions during the past 5 years (9). In this study, the highest prevalence of ESBL producers, which amounted to 15%, was observed with non-E. coli non-Klebsiella Enterobacteriaceae (Table 1). This is of concern, since spread of resistance among these species is known to occur through plasmids and these species are therefore particularly prone to spread and cause outbreaks (4, 9, 10, 11, 15). To date, SHV-type ESBLs dominated in surveys of resistant clinical isolates in Europe and North America (12, 20). This was also the case in one of the participating hospitals in Amsterdam (AMC) in 2000 (9). In 2003, CTX-M ESBL genes were detected in the AMC for the first time, and the present study shows the dissemination of four different CTX-M ESBLs in nine different species, including the nonfermenting gram-negative species (Table 1). This wide occurrence is remarkable. Pitout et al. (14) reported the spread of Enterobacteriaceae carrying different CTX-M ESBLs, originating from the community. Possibly, a similar phenomenon is occurring in Amsterdam. However, we cannot exclude the possibility of the spread of these different groups of CTX-M enzymes between the medical centers or through other routes, e.g., patient transfer.
The emergence and the increase in the prevalence of CTX-M ESBLs is remarkable and may be correlated with the increased use of cefotaxime, which has been suggested to play an important role in the selection for CTX-M ESBLs (13, 19). The gentamicin resistance rate in ESBL producers in this study was high compared to that found among all gram-negative isolates from patients in Dutch hospitals (6) and confirms that the use of non--lactam antibiotics for treatment of infections caused by ESBL-producing pathogens is limited and that only a few agents, such as carbapenems, provide solid coverage. More rational analysis of antibiotic policies and of risk factors for carriage of ESBL-producing pathogens among patients is required.
The present study suggests that ESBLs are a growing problem in Amsterdam and that the establishment of these baseline data is crucial for the evaluation of the epidemiology of ESBLs and the antibiotic policy in this region.
ACKNOWLEDGMENTS
N. al Naiemi participated in the phenotypic and molecular typing of strains and genes. N. al Naiemi, A. Bart, M. D. de Jong, C. M. Vandenbroucke-Grauls, and B. Duim participated in the development of a research strategy, directed the analyses, and led the writing of the manuscript.
P. C. Wever, Y. J. Debets-Ossenkopp, P. J. G. M. Rietra, L. Spanjaard, and A. J. Bos participated in collection and identification of the bacterial isolates. All authors appraised and approved the final report.
The authors do not have a commercial or other association that might pose a conflict of interest. This study was performed without external financial support.
FOOTNOTES
Corresponding author. Mailing address: Dept. of Medical Microbiology, Academic Medical Center, L1-244, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Phone: 31 20 5665714. Fax: 31 20 5669745. E-mail: b.duim@amc.uva.nl.
Current address: Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.
REFERENCES
Albertini, M. T., C. Benoit, L. Berardi, Y. Berrouane, A. Boisivon, P. Cahen, C. Cattoen, Y. Costa, P. Darchis, E. Deliere, D. Demontrond, F. Eb, F. Golliot, G. Grise, A. Harel, J. L. Koeck, M. P. Lepennec, C. Malbrunot, M. Marcollin, S. Maugat, M. Nouvellon, B. Pangon, S. Ricouart, M. Roussel-Delvallez, A. Vachee, A. Carbonne, L. Marty, V. Jarlier, et al. 2002. Surveillance of methicillin-resistant Staphylococcus aureus (MRSA) and Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBLE) in Northern France: a five-year multicentre incidence study. J. Hosp. Infect. 52:107-113.
Bradford, P. A. 2001. Extended-spectrum -lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev. 14:933-951.
Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing; 15th informational supplement (M100-S15). Clinical and Laboratory Standards Institute, Wayne, Pa.
Dumarche, P., C. De Champs, D. Sirot, C. Chanal, R. Bonnet, and J. Sirot. 2002. TEM derivative-producing Enterobacter aerogenes strains: dissemination of a prevalent clone. Antimicrob. Agents Chemother. 46:1128-1131.
Goossens, H. 2001. MYSTIC program: summary of European data from 1997 to 2000. Diagn. Microbiol. Infect. Dis. 41:183-189.
Kaiser, A. M., C. Schultsz, G. J. Kruithof, Y. Debets-Ossenkopp, and C. Vandenbroucke-Grauls. 2004. Carriage of resistant microorganisms in repatriates from foreign hospitals to The Netherlands. Clin. Microbiol. Infect. 10:972-979.
Marchandin, H., C. Carriere, D. Sirot, H. J. Pierre, and H. Darbas. 1999. TEM-24 produced by four different species of Enterobacteriaceae, including Providencia rettgeri, in a single patient. Antimicrob. Agents Chemother. 43:2069-2073.
Naas, T., L. Philippon, L. Poirel, E. Ronco, and P. Nordmann. 1999. An SHV-derived extended-spectrum -lactamase in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 43:1281-1284.
Naiemi, N. A., B. Duim, P. H. Savelkoul, L. Spanjaard, E. de Jonge, A. Bart, C. M. Vandenbroucke-Grauls, and M. D. de Jong. 2005. Widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology. J. Clin. Microbiol. 43:4862-4864.
Neuwirth, C., E. Siebor, J. Lopez, A. Pechinot, and A. Kazmierczak. 1996. Outbreak of TEM-24-producing Enterobacter aerogenes in an intensive care unit and dissemination of the extended-spectrum beta-lactamase to other members of the family Enterobacteriaceae. J. Clin. Microbiol. 34:76-79.
Palucha, A., B. Mikiewicz, W. Hryniewicz, and M. Gniadkowski. 1999. Concurrent outbreaks of extended-spectrum beta-lactamase-producing organisms of the family Enterobacteriaceae in a Warsaw hospital. J. Antimicrob. Chemother. 44:489-499.
Paterson, D. L., K. M. Hujer, A. M. Hujer, B. Yeiser, M. D. Bonomo, L. B. Rice, R. A. Bonomo, and the International Klebsiella Study Group. 2003. Extended-spectrum -lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV- and CTX-M-type -lactamases. Antimicrob. Agents Chemother. 47:3554-3560.
Patterson, J. E. 2001. Antibiotic utilization: is there an effect on antimicrobial resistance Chest 119:426S-430S.
Pitout, J. D., D. B. Gregson, D. L. Church, S. Elsayed, and K. B. Laupland. 2005. Community-wide outbreaks of clonally related CTX-M-14 -lactamase-producing Escherichia coli strains in the Calgary health region. J. Clin. Microbiol. 43:2844-2849.
Pitout, J. D., K. S. Thomson, N. D. Hanson, A. F. Ehrhardt, P. Coudron, and C. C. Sanders. 1998. Plasmid-mediated resistance to expanded-spectrum cephalosporins among Enterobacter aerogenes strains. Antimicrob. Agents Chemother. 42:596-600.
Sirot, J., M. H. Nicolas-Chanoine, H. Chardon, J. L. Avril, C. Cattoen, J. C. Croix, H. Dabernat, T. Fosse, J. C. Ghnassia, E. Lecaillon, A. Marmonier, M. Roussel-Delvallez, C. J. Soussy, A. Trevoux, F. Vandenesch, C. Dib, N. Moniot-Ville, and Y. Rezvani. 2002. Susceptibility of Enterobacteriaceae to beta-lactam agents and fluoroquinolones: a 3-year survey in France. Clin. Microbiol. Infect. 8:207-213.
Stobberingh, E. E., J. Arends, J. A. Hoogkamp-Korstanje, W. H. Goessens, M. R. Visser, A. G. Buiting, Y. J. Debets-Ossenkopp, R. J. van Ketel, M. L. van Ogtrop, L. J. Sabbe, G. P. Voorn, H. L. Winter, and J. H. van Zeijl. 1999. Occurrence of extended-spectrum betalactamases (ESBL) in Dutch hospitals. Infection 27:348-354.
Sturenburg, E., and D. Mack. 2003. Extended-spectrum beta-lactamases: implications for the clinical microbiology laboratory, therapy, and infection control. J. Infect. 47:273-295.
Wang, H., S. Kelkar, W. Wu, M. Chen, and J. P. Quinn. 2003. Clinical isolates of Enterobacteriaceae producing extended-spectrum -lactamases: prevalence of CTX-M-3 at a hospital in China. Antimicrob. Agents Chemother. 47:790-793.
Yuan, M., H. Aucken, L. M. Hall, T. L. Pitt, and D. M. Livermore. 1998. Epidemiological typing of klebsiellae with extended-spectrum beta-lactamases from European intensive care units. J. Antimicrob. Chemother. 41:527-539.(N. al Naiemi, A. Bart, M. D. de Jong, C.)