己烯雌酚建立尿道下裂大鼠模型的实验研究.PDF
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·实验研究·
作者单位: 610041 成都, 四川大学华西医院泌尿外科(龚春
雨、李虹、魏强、罗琦、李响) ,四川大学华西医院小儿外科泌尿组(黄
鲁刚)
己烯雌酚建立尿道下裂大鼠模型的实验研究
龚春雨 黄鲁刚 李虹 魏强 罗琦 李响
【摘要】 目的 尝试用己烯雌酚(DES)建立尿道下裂大鼠模型。方法 孕鼠 20 只 ,随机分成 2
组:生理盐水对照组(CG) 10 只 , DES组10 只。在大鼠怀孕12～17 d期间 ,每组每天分别给予管喂生
理盐水1. 5 ml/ d ,DES 100μg· kg -
· d - 1。待孕大鼠分娩完毕 ,即对新生大鼠计数并在放大镜及解剖显
微镜下观察雄性新生鼠的阴茎包皮外观、尿道口位置、排尿情况 , 测量肛生殖距离(AGD)和称体重。
结果 ①DES成功诱导出了雄性大鼠尿道下裂畸形 ,对照组大鼠的生殖器未见异常改变; ②DES组
新生大鼠的 AGD缩短 ,但无统计学差异 , DES组体重明显减轻 ,有显著统计学差异。结论 ①成功
建立的尿道下裂大鼠模型 ,较国外尿道下裂小鼠更直观 ,更规范; ②尿道下裂大鼠模型可以为研究尿
道下裂病因学和防治措施提供基础。
【关键词】 尿道下裂; 疾病模型 ,动物; 己烯雌酚
The establ ishment of hypospadias rat model with diethylstilbestrol GON G Chun2yu , HUA N G L u2
gang , L I Hong , W EI Qiang , L UO Qi , L I Xiang. Department of Urology , West China Hospi tal of
Sichuan Universi ty , Chengdu 610041 , China
【Abstract】 Objective To induce hypospadias in rats by diethylstilbest rol (DES) . Methods 20
conceived rats were divided randomly into 2 groups : 10 with saline for cont rol (CG) and 10 with DES.
From 12th to 17th day after the day of conception of the female rats , the rats in CGgroup were feed with
1. 5 ml saline and 100μg/ kg/ d DES in the t reatment group. After all pregnant rats had delivered , the male
newborn rats were counted and the penis appearance , urethral orifice position and micturation were obse2
rved with magnifying lens and anatomy microscope. The anal genital distance (AGD) and body weight we2
re also measured. Results (1) Hypospadias was found in newborn male rats t reated prenatally with DES ,while there was no urethral orifice abnormality observed in the CG. (2) DES shortened the AGD of new2
born rats , but there was no statistical differences between the 2 groups. The lightening of weight on new2
born rats t reated with DES showed significant statistic differences. Conclusions (1) A hypospadias rat
model has been established and it is easily reproducible and its appearance is very much like the real human
hypospadias. (2) The hypospadias rat model can offer the base for further research.
【Key words】 Hypospadias ; Disease models ,animal ; Diethylstilbest rol
尿道下裂是小儿外科常见的先天性畸形之一 ......
作者单位: 610041 成都, 四川大学华西医院泌尿外科(龚春
雨、李虹、魏强、罗琦、李响) ,四川大学华西医院小儿外科泌尿组(黄
鲁刚)
己烯雌酚建立尿道下裂大鼠模型的实验研究
龚春雨 黄鲁刚 李虹 魏强 罗琦 李响
【摘要】 目的 尝试用己烯雌酚(DES)建立尿道下裂大鼠模型。方法 孕鼠 20 只 ,随机分成 2
组:生理盐水对照组(CG) 10 只 , DES组10 只。在大鼠怀孕12～17 d期间 ,每组每天分别给予管喂生
理盐水1. 5 ml/ d ,DES 100μg· kg -
· d - 1。待孕大鼠分娩完毕 ,即对新生大鼠计数并在放大镜及解剖显
微镜下观察雄性新生鼠的阴茎包皮外观、尿道口位置、排尿情况 , 测量肛生殖距离(AGD)和称体重。
结果 ①DES成功诱导出了雄性大鼠尿道下裂畸形 ,对照组大鼠的生殖器未见异常改变; ②DES组
新生大鼠的 AGD缩短 ,但无统计学差异 , DES组体重明显减轻 ,有显著统计学差异。结论 ①成功
建立的尿道下裂大鼠模型 ,较国外尿道下裂小鼠更直观 ,更规范; ②尿道下裂大鼠模型可以为研究尿
道下裂病因学和防治措施提供基础。
【关键词】 尿道下裂; 疾病模型 ,动物; 己烯雌酚
The establ ishment of hypospadias rat model with diethylstilbestrol GON G Chun2yu , HUA N G L u2
gang , L I Hong , W EI Qiang , L UO Qi , L I Xiang. Department of Urology , West China Hospi tal of
Sichuan Universi ty , Chengdu 610041 , China
【Abstract】 Objective To induce hypospadias in rats by diethylstilbest rol (DES) . Methods 20
conceived rats were divided randomly into 2 groups : 10 with saline for cont rol (CG) and 10 with DES.
From 12th to 17th day after the day of conception of the female rats , the rats in CGgroup were feed with
1. 5 ml saline and 100μg/ kg/ d DES in the t reatment group. After all pregnant rats had delivered , the male
newborn rats were counted and the penis appearance , urethral orifice position and micturation were obse2
rved with magnifying lens and anatomy microscope. The anal genital distance (AGD) and body weight we2
re also measured. Results (1) Hypospadias was found in newborn male rats t reated prenatally with DES ,while there was no urethral orifice abnormality observed in the CG. (2) DES shortened the AGD of new2
born rats , but there was no statistical differences between the 2 groups. The lightening of weight on new2
born rats t reated with DES showed significant statistic differences. Conclusions (1) A hypospadias rat
model has been established and it is easily reproducible and its appearance is very much like the real human
hypospadias. (2) The hypospadias rat model can offer the base for further research.
【Key words】 Hypospadias ; Disease models ,animal ; Diethylstilbest rol
尿道下裂是小儿外科常见的先天性畸形之一 ......
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